Tissue Arraying, Immunostaining, and Imaging System

描述（由申请人提供）：Fox Chase癌症中心（FCCC）需要一个用于组织排列、自动免疫染色和细胞/组织图像分析的系统来增强现有的两个核心设施。所要求的设备将允许无缝提供服务，包括组织微阵列（TMA）的构建，TMA和常规组织学切片的自动免疫染色以及使用计算机辅助图像分析仪的免疫组织化学反应的定量。该系统将由肿瘤库设施和癌症中心核心基金（P-30）支持的组织病理学设施管理。核心设施由经验丰富的病理学家指导，他们共享设备和实验室空间，以提高服务效率。自1990年成立以来，肿瘤库已经积累了超过12000份石蜡包埋的肿瘤和非肿瘤人体组织标本，这些标本来自16个器官部位的经同意的手术病例。这些组织为癌症生物学、癌变发生、靶向化疗和癌症预防计划的分子、生化和细胞研究提供了宝贵的资源。组织病理学设施为使用实验动物和人类模型的研究人员提供组织病理学和免疫组织化学服务。这两个机构密切合作，协同工作，为FCCC调查人员提供最好的病理支持。所要求的共享设备将更新和增强30多名首席研究员使用的病理学相关设施。所要求的高级组织阵列将允许来自同一标本的组织的高效多用户分布，提供感兴趣的组织学/病理学的确切类型。Dako Autostainer将加快大量样品的免疫组织化学染色过程。然后，染色结果将由自动细胞图像系统III进行分析，以实现数据的精确定量分析，避免目前主观的半定量评分方法。该提案将使FCCC众多正在进行和准备开发的项目受益。所有这些研究项目产生的数据将为乳腺癌和舌癌的化学预防、结直肠癌的化疗、锌在前列腺癌发生中的作用、肾癌的DNA甲基化以及各种假定的癌症生物标志物的验证提供有用的信息。

DESCRIPTION (provided by applicant): A system for tissue arraying, automatic immunostaining and cell/tissue image analysis is requested to enhance two existing Core Facilities at Fox Chase Cancer Center (FCCC). The requested equipment will permit a seamless provision of services including construction of tissue microarrays (TMA), automatic immunostaining of TMA and regular histological sections as well as quantitation of the immunohistochemical reactions using a computer-assisted image analyzer. This system will be managed by the Tumor Bank Facility and the Histopathology Facility supported by Cancer Center Core Grant (P-30). The Core facilities are directed by experienced pathologists who share equipment and laboratory space to enhance the efficiency of the services. The Tumor Bank has accrued over 12,000 specimens of paraffin embedded neoplastic and non-neoplastic human tissues from consented surgical cases from 16 organ sites since its inception in 1990. These tissues provide precious resources for molecular, biochemical and cellular studies for the cancer biology, carcinogenesis, targeted chemotherapy and cancer prevention programs. The Histopathology Facility provides histopathology and immunohistochemistry services to investigators using experimental animal and human models. These two facilities work in close collaboration and synergize to provide the best possible pathology support to FCCC investigators. The requested shared equipment will update and enhance both pathology-associated facilities used by more than 30 Principal Investigators. The requested Advanced Tissue Arrayer will permit an efficient multi-user distribution of tissues from the same specimen, providing the exact type of histology/pathology of interest. Dako Autostainer will expedite the immunohistochemical staining process for a large number of samples. The staining results will then be analyzed by the Automated Cellular Image System III, to achieve precise, quantitative analyses of the data, avoiding the current subjective, semi-quantitative scoring method. This proposal will benefit numerous ongoing and ready to be developed projects at FCCC. Data generated from all these research projects will provide useful information in chemoprevention of breast cancer and tongue cancer, colorectal cancer chemotherapy, role of zinc in prostate carcinogenesis, DNA methylation in renal cancers, and validation of various putative cancer biomarkers.