Tissue Arraying, Immunostaining, and Imaging System

描述(由申请人提供)：需要一个用于组织排列、自动免疫染色和细胞/组织图像分析的系统，以增强狐狸大通癌症中心(Fccc)现有的两个核心设施。所要求的设备将允许无缝提供服务，包括组织微阵列的构建、组织微阵列和常规组织切片的自动免疫染色以及使用计算机辅助图像分析仪对免疫组织化学反应进行量化。该系统将由肿瘤库设施和由癌症中心核心赠款(P-30)支持的组织病理学设施管理。核心设施由经验丰富的病理学家指导，他们共用设备和实验室空间，以提高服务效率。自1990年成立以来，肿瘤库已经从16个器官部位收集了超过12000份石蜡包埋的肿瘤和非肿瘤人体组织样本，这些组织来自同意的手术病例。这些组织为癌症生物学、癌症发生、靶向化疗和癌症预防计划的分子、生化和细胞研究提供了宝贵的资源。组织病理学机构使用实验动物和人体模型为研究人员提供组织病理学和免疫组织化学服务。这两个机构密切合作，协同工作，为fccc的研究人员提供尽可能好的病理学支持。所要求的共享设备将更新和加强30多名首席调查员使用的与病理学有关的设施。所要求的先进组织排列器将允许多用户有效地分配来自同一标本的组织，提供感兴趣的组织学/病理学的确切类型。DAKO AutoStander将加快大量样本的免疫组织化学染色过程。染色结果将由自动细胞图像系统III进行分析，以实现对数据的精确、定量分析，避免了目前主观的、半定量的评分方法。这项提议将使“气候变化框架公约”的许多正在进行和随时准备开发的项目受益。所有这些研究项目产生的数据将在乳腺癌和舌癌的化学预防、结直肠癌的化疗、锌在前列腺癌发生中的作用、肾癌的DNA甲基化以及各种假定的癌症生物标志物的验证方面提供有用的信息。

DESCRIPTION (provided by applicant): A system for tissue arraying, automatic immunostaining and cell/tissue image analysis is requested to enhance two existing Core Facilities at Fox Chase Cancer Center (FCCC). The requested equipment will permit a seamless provision of services including construction of tissue microarrays (TMA), automatic immunostaining of TMA and regular histological sections as well as quantitation of the immunohistochemical reactions using a computer-assisted image analyzer. This system will be managed by the Tumor Bank Facility and the Histopathology Facility supported by Cancer Center Core Grant (P-30). The Core facilities are directed by experienced pathologists who share equipment and laboratory space to enhance the efficiency of the services. The Tumor Bank has accrued over 12,000 specimens of paraffin embedded neoplastic and non-neoplastic human tissues from consented surgical cases from 16 organ sites since its inception in 1990. These tissues provide precious resources for molecular, biochemical and cellular studies for the cancer biology, carcinogenesis, targeted chemotherapy and cancer prevention programs. The Histopathology Facility provides histopathology and immunohistochemistry services to investigators using experimental animal and human models. These two facilities work in close collaboration and synergize to provide the best possible pathology support to FCCC investigators. The requested shared equipment will update and enhance both pathology-associated facilities used by more than 30 Principal Investigators. The requested Advanced Tissue Arrayer will permit an efficient multi-user distribution of tissues from the same specimen, providing the exact type of histology/pathology of interest. Dako Autostainer will expedite the immunohistochemical staining process for a large number of samples. The staining results will then be analyzed by the Automated Cellular Image System III, to achieve precise, quantitative analyses of the data, avoiding the current subjective, semi-quantitative scoring method. This proposal will benefit numerous ongoing and ready to be developed projects at FCCC. Data generated from all these research projects will provide useful information in chemoprevention of breast cancer and tongue cancer, colorectal cancer chemotherapy, role of zinc in prostate carcinogenesis, DNA methylation in renal cancers, and validation of various putative cancer biomarkers.