Generation /Analysis of Mice Carrying GFP-tagged Genes in Kidney Development

描述（由申请人提供）： 哺乳动物肾脏的发育是一个非常复杂的过程，包括输尿管的分支形态发生和肾单位的形成。一旦肾单位形成，下一个发育阶段是Henle袢和集合管延伸到肾盂，形成髓质。阐明肾脏中基因的时空表达模式对于了解肾脏的器官发生和生理功能至关重要。因此，我们建议分析100个基因在发育中的肾脏中的表达模式。这些基因要么是新的，要么尚未报道在肾脏中表达或发挥功能。我们将用GFP标记这些基因以可视化时空表达模式。对这些小鼠的分析将使我们能够：1）确定基因功能丧失对肾脏发育的影响; 2）跟踪纯合突变细胞的命运，因为它们现在被GFP标记;以及3）通过可视化GFP标签来确定基因产物的亚细胞定位，这通常非常揭示基因的功能

DESCRIPTION (provided by applicant): The development of the mammalian kidney is a very complex process, involving branching morphogenesis of the ureter and the formation of nepherons. Once the nepheron is formed, the next stage of development is for the loop of Henle and collecting duct to elongate into the renal pelvis, forming the medulla. Delineation of the spatial and temporal expression patterns of genes in kidney is essential for understanding its organogenesis and physiological function. Therefore, we proposed to analyze the expression patterns of 100 genes in the developing kidney. These genes are either novel or have not been reported to be expressed or to be functioning in the kidney. We will tag these genes with GFP to visualize the spatio-temporal expression patterns. Analyses of these mice will be enable us to: 1) determine the effect of the loss of the gene function on renal development; 2) follow the fate of the homozygous mutant cells as they are now marked by GFP; and 3) determine the subcellular localization of the gene product by visualizing the GFP tag, which often time is very revealing about a gene's function