Morphology and Molecular

形态和分子生物学核心将为该计划中的各种项目提供 执行常规形态和分子生物学所需的专业知识、设施和支持 程序。培训技术人员、研究生和博士后研究员是 核心C由核心C的形态组件提供的服务包括常规光学显微镜， 免疫荧光显微镜和电子显微镜。核心员工将协助收购和 形态数据的解释。其他特殊的形态和免疫组织化学支持将 根据需要进行开发。Core C的这一组成部分将支持该计划中的所有项目。这些服务 由核心C的分子生物学部分提供的将包括DNA的生长和纯化以及 重组病毒；cDNA定点突变，转基因载体和质粒的生产， 通道蛋白和连接蛋白在哺乳动物细胞培养中的表达、分析及原代生产 新生心肌细胞的培养。当需要时，将设计和测试RNA沉默构造 在使用之前由内核提供。核心也将是生产和维护的主要设施 转基因小鼠群体。这些职责将包括育种、基因分型和表型分析。 通过免疫化学手段。核心C的这一组成部分将主要支持项目2和3。

The Morphology and Molecular Biology Core will provide the various projects in the program with the necessary expertise, facilities, and support to perform routine morphological and molecular biological proceedures. Training of technicians, graduate students and post-doctoral fellows is an integral aspect of Core C. The services provided by the Morphology component of Core C include routine light microscopy, immunofluorescent microscopy and electron microscopy. Core staff will assist in both the acquisition and interpretation of morphological data. Other specialized morphological and immunohistochemical support will be developed as required. This component of Core C will support all projects in the program. The services provided by the Molecular Biology component of Core C will include the growth and purification of DNA and recombinant viruses; site directed mutagenesis of cDNA, production of transfection vectors and plasmids, expression, analysis of channel and junction proteins in mammalian cell cultures and production of primary cultures of neonatal cardiac myocytes. When needed, RNA silencing constructs will be designed and tested by the Core prior to use. The Core will also be the primary facility for the production and maintenance of the transgenic mouse colonies. These responsibilities will include breeding, genotyping and phenotypic analysis by immunochemical means. This component of Core C will primarily support projects 2 and 3.