CORE--Cell Separation and Quantative Transplantation

细胞分离和定量移植核心将支持这方面的研究人员和项目 计划项目赠款(PPG)，通过提供高度浓缩的细胞群体用于造血干细胞和 用于体外和体内实验的未成熟前体细胞；通过提供多参数流式细胞术 分析来自移植动物和体外培养的细胞；并通过进行竞争性和 量化移植研究。核心将执行细胞染色、细胞分离、细胞分类和 用流式细胞术分析研究人员对PPG的影响。核心将进行体内移植 研究人员对生产的细胞群体的长期骨髓再生功能进行量化的研究 通过不同的项目。为了便于分析捐赠者细胞的骨髓再繁殖情况，Core将使用 合适的同源品系(例如CD45.1和CD45.2)的移植受体，其中供体细胞可以 很容易从竞争或残留的宿主细胞中区分出来。核心将进行所有的移植， 在移植后对动物进行照射、输血和维持，并为所有 PPG的调查人员。中心将对移植动物的细胞进行分析，以量化捐赠者 细胞在不同造血系中的植入。核心通过以下方式提供资源的高效利用 能够为多个项目分离统一的细胞群体，并在 同时，通过协调项目之间的移植研究，使共同的控制组 动物被使用，从而减少了动物的使用。

The Cell Separation and Quantitative Transplantation Core will support investigators and projects on this Program Project Grant (PPG) by providing cell populations highly enriched for hematopoietic stem and immature precursor cells for in vitro and in vivo experiments; by providing multi-parameter flow cytometric analysis of cells from transplanted animals and in vitro cultures; and by performing competitive and quantitative transplantation studies. The Core will perform cell staining, cell separations, cell sorting and analysis using flow cytometry for investigators on the PPG. The Core will perform in vivo transplantation studies for investigators to quantify the long-term marrow repopulating function of cell populations produced by the different Projects. To facilitate analysis of marrow repopulation by donor cells the Core will use transplant recipients of the appropriate congenic strains (e.g. CD45.1 vs. CD45.2) in which donor cells can easily be distinguished from competing or residual host cells. The core will perform all the transplants, irradiating, infusing and maintaining animals after transplant as well as collecting specimens for all the investigators in the PPG. The Core will perform analysis of cells from transplanted animals to quantify donor cell engraftment in different hematopoietic lineages. The Core provides an efficient use of resources by being able to isolate uniform populations of cells for multiple projects and collaborating investigators at the same time, and by coordinating transplantation studies between projects so that common groups of control animals are used, thereby decreasing animal usage.