CORE--CELL /MOLECULAR /MICROSCOPY

分子遗传学和蛋白质组学核心作为一种资源，提供专业知识和 协助Van Eyk和Tomaselli的项目分析基因和蛋白质表达。核心的重点将是用于微阵列和蛋白质表达研究的性能和分析的信使核糖核酸和蛋白质的制备和利用。核心设施的活动将在约翰霍普金斯医学院校园内的几个地点进行，包括心脏病学部、西德尼·基梅尔癌症综合护理微阵列设施以及生物医学工程系中心的计算医学和生物学中心的实验室。该中心提供的服务包括：动态实时聚合酶链式反应(RT-PCR)和微阵列实验的信使核糖核酸的制备和鉴定；获得犬EST文库；识别和杂交微阵列，协助分析微阵列数据；获得CAGE，微阵列实验的数据库；RT-PCR的引物；犬组织合格的抗体及其在蛋白质印迹、免疫组织化学和免疫细胞化学应用中的应用；胞浆、肌膜、肌浆网、ERK1/2和连接蛋白亚组的制备和分析(包括2D凝胶电泳(2DE)、2D液相色谱(2DLC)、差异成像凝胶电泳(DICE)和质谱仪)；以及用于多肽质量指纹分析(PMF)的犬心脏特异蛋白质数据库。除了为这些项目提供技术专长外，该核心还将作为犬心脏的分子生物和蛋白质化学分析的集中资源，从而节省大量成本。

The molecular genetics and proteomics core serves as a resource to provide expertise and assistance in the analysis of gene and protein expression to projects by Van Eyk and Tomaselli. The emphasis of the core will be on the preparation and utilization of mRNA and protein for the performance and analysis of microarray and protein expression studies. Activities of the core facility will be performed in several locations on the campus of the Johns Hopkins School of Medicine including laboratories in the Division of Cardiology, the Sidney Kimmel Comprehensive Cancer Care Microarray Facility and the Center for Computational Medicine and Biology centered in the Department of Biomedical Engineering. The services provided by this Core include: mRNA preparation and qualification for kinetic real time PCR (RT-PCR) and microarray experiments; access to canine EST libraries; spotting and hybridization to microarrays, assistance with analysis of microarray data; access to CAGE, a database repository for microarray experiments; primers for RT-PCR; canine tissue qualified antibodies and protocols for their use in Western blotting, immunohistochemical and immunocytochemical applications; cytosolic, sarcolemmal, sarcoplasmic reticulum, ERK1/2 and connexin subproteome preparation and analysis (including the performance of 2D gel electrophoresis (2DE), 2D liquid chromatography (2DLC), differential imaging gel electrophoresis (DICE) and mass spectrometry); and a canine heart-specific protein database for peptide mass fingerprinting (PMF). In addition to the provision of technical expertise to the projects, the core will generate significant cost savings by serving as a centralized resource for molecular biological and protein chemical analysis of the canine heart.