MASS SPECTROMETRY CORE

质谱学核心的目的是通过量化的方式为项目提供支持 LC/MS/MS法是测定类固醇的主要方法 活动。此外，专门的服务将包括用凝胶分离的蛋白质的鉴定 钙通道/受体亚型及其氧化敏感性的电泳法、鉴定和定量 基于电喷雾电离(ESI)的方法研究对功能至关重要的半胱氨酸(Cys)硫醇 LC/MS/MS这些支持需求由项目负责人确定，要求/方法是 通过与核心总监的互动详细讨论，以选择最合适的 作为核心服务实施。共享基础上可用的设备是四极离子陷阱 用于类固醇分析的质谱仪和线性离子陷阱-傅里叶变换-离子回旋共振 用于蛋白质组学相关工作的仪器。该系统还结合了先进的液体色谱图 样本分色。核心B将提供专家领导、专家和技术人员来执行核心 并提供关于类固醇定量分析和蛋白质鉴定结果的完整报告， 对项目领导和研究人员进行量化。

The purpose of the Mass Spectrometry Core is to provide support for the projects by quantitative determination of steroids by liquid chromatography - tandem mass spectrometry (LC/MS/MS) as its major activity. Additionally, specialized services will include identification of proteins separated by gel electrophoresis, identification and quantification of Ca2+-channel/receptor isotypes and their oxidationsensitive cysteine (Cys) thiols critical to function by methods based on electrospray ionization (ESI) and LC/MS/MS. These support needs were identified by the project leaders, and the requirements/methods were discussed in details through interactions with the core director to select the most appropriate ones to be implemented as core services. Equipments made available on a shared basis are a quadrupole ion-trap mass spectrometer for steroid analysis and a linear ion-trap - Fourier-transform ion-cyclotron resonance instrument for proteomics-related work. The systems also incorporate advanced liquid chrom atographs for sample separations. Core B will provide expert leadership, specialists and technical staff to perform the core services and provide full reports on the results of quantitative steroid analyses and on protein identification, quantification to the project leaders and research personnel.