MASS SPECTROMETRY CORE

质谱核心

• 批准号: 7246200
• 负责人: LASZLO PROKAI
• 金额: $ 11.61万
• 依托单位: UNIVERSITY OF NORTH TEXAS HLTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-15 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7246200
• 关键词: Cyclotrons; Cysteine; Electrospray Ionization; Equipment; Fourier Transform; Ions; Leadership; Liquid Chromatography; Liquid substance; Mass Spectrum Analysis; Methods; Proteins; Proteomics; Purpose; Reporting; Research Personnel; Sampling; Services; Specialist; Steroids; Sulfhydryl Compounds; System; Work; base; gel electrophoresis; instrument; mass spectrometer; oxidation; receptor; tandem mass spectrometry

The purpose of the Mass Spectrometry Core is to provide support for the projects by quantitative determination of steroids by liquid chromatography - tandem mass spectrometry (LC/MS/MS) as its major activity. Additionally, specialized services will include identification of proteins separated by gel electrophoresis, identification and quantification of Ca2+-channel/receptor isotypes and their oxidationsensitive cysteine (Cys) thiols critical to function by methods based on electrospray ionization (ESI) and LC/MS/MS. These support needs were identified by the project leaders, and the requirements/methods were discussed in details through interactions with the core director to select the most appropriate ones to be implemented as core services. Equipments made available on a shared basis are a quadrupole ion-trap mass spectrometer for steroid analysis and a linear ion-trap - Fourier-transform ion-cyclotron resonance instrument for proteomics-related work. The systems also incorporate advanced liquid chrom atographs for sample separations. Core B will provide expert leadership, specialists and technical staff to perform the core services and provide full reports on the results of quantitative steroid analyses and on protein identification, quantification to the project leaders and research personnel.

质谱核心的目的是通过定量分析为项目提供支持。 以液相色谱-串联质谱法（LC/MS/MS）测定甾体类化合物为主要方法， 活动此外，专业服务将包括鉴定凝胶分离的蛋白质 钙通道/受体同种型的电泳、鉴定和定量及其氧化敏感性 通过基于电喷雾电离（ESI）的方法， LC/MS/MS。这些支持需求由项目负责人确定，要求/方法如下： 通过与核心主任的互动进行详细讨论，以选择最合适的方案， 作为核心服务。在共享基础上提供的设备是四极离子阱 甾族化合物质谱仪和线性离子阱-傅里叶变换离子回旋共振 用于蛋白质组学相关工作的仪器。该系统还包括先进的液相色谱仪， 样品分离核心B将提供专家领导、专家和技术人员来执行核心 服务，并提供有关定量类固醇分析结果和蛋白质鉴定的完整报告， 量化到项目负责人和研究人员。