CORE--GENE EXPRESSION

这个核心的目的是通过实时聚合酶链式反应技术向参与该计划项目的研究人员提供高效、质量控制和成本效益高的基因表达量化。基因表达核心将为Rossetti的项目量化下丘脑神经肽的表达，并为Barzilai、Hawkins、Brownlee和ShaMoon的项目量化脂肪衍生和细胞因子。实时聚合酶链式反应对基因表达的定量将集中在一个单一的设施中，并可供该项目的研究人员使用。该核心人员将负责对每个实验进行实时聚合酶链式反应和质量控制测试。他们 还将协助设计新的特殊底漆。这个核心将由奥比奇博士指导。 安排运行测试的优先顺序将由参与者同意后决定 程序。

The purpose of this Core is to provide efficient, quality-controlled, and cost-effective quantitation of gene expression by real-time PCR techniques to the investigators participating in this Program Project. The gene expression core will quantify hypothalamic neuropeptide expression for project by Rossetti, and fat-derived and cytokines for projects by Barzilai, Hawkins, Brownlee, and Shamoon. The quantitation of gene expression by real-time PCR will be centralized in a single facility and available to the investigators of this program. The personnel of this core will be responsible for running real time PCR and quality control testing of each experiment. They will also assist for the design of new speific primers. This core will be directed by Dr. Obici. Scheduling the priority for running tests will be decided by consent among the participants of the program.