CORE--GENE EXPRESSION

该核心的目的是通过实时PCR技术为参与该计划项目的研究人员提供高效，质量受控和具有成本效益的基因表达定量。基因表达核心将量化Rossetti项目的下丘脑神经肽表达，以及Barzilai，Hawkins，Brownlee和Shamoon项目的脂肪衍生和细胞因子。通过实时PCR进行的基因表达定量将集中在一个设施中，并可供本项目的研究者使用。该中心的人员将负责运行真实的时间PCR和每次实验的质量控制检测。他们 也将有助于设计新的特异性引物。这个核心将由Obici博士指导。 安排运行测试的优先级将由参与者的同意决定， 程序.

The purpose of this Core is to provide efficient, quality-controlled, and cost-effective quantitation of gene expression by real-time PCR techniques to the investigators participating in this Program Project. The gene expression core will quantify hypothalamic neuropeptide expression for project by Rossetti, and fat-derived and cytokines for projects by Barzilai, Hawkins, Brownlee, and Shamoon. The quantitation of gene expression by real-time PCR will be centralized in a single facility and available to the investigators of this program. The personnel of this core will be responsible for running real time PCR and quality control testing of each experiment. They will also assist for the design of new speific primers. This core will be directed by Dr. Obici. Scheduling the priority for running tests will be decided by consent among the participants of the program.