CORE--GENE EXPRESSION

本核心的目的是通过实时PCR技术为参与本项目的研究人员提供高效、质量控制和成本效益的基因表达定量。基因表达核心将量化Rossetti项目的下丘脑神经肽表达，以及Barzilai、Hawkins、Brownlee和Shamoon项目的脂肪源性和细胞因子。通过实时PCR的基因表达定量将集中在一个单一的设施，并提供给该计划的研究人员。该核心的人员将负责运行实时PCR和每个实验的质量控制测试。他们

The purpose of this Core is to provide efficient, quality-controlled, and cost-effective quantitation of gene expression by real-time PCR techniques to the investigators participating in this Program Project. The gene expression core will quantify hypothalamic neuropeptide expression for project by Rossetti, and fat-derived and cytokines for projects by Barzilai, Hawkins, Brownlee, and Shamoon. The quantitation of gene expression by real-time PCR will be centralized in a single facility and available to the investigators of this program. The personnel of this core will be responsible for running real time PCR and quality control testing of each experiment. They will also assist for the design of new speific primers. This core will be directed by Dr. Obici. Scheduling the priority for running tests will be decided by consent among the participants of the program.