CORE--Proteomics Facility

描述(由申请人提供)：蛋白质组学设施核心旨在开发、采用和维护蛋白质组学研究的最先进的专业知识和设备，并提供针对外源代谢酶及其转运体的独特抗体，供中心成员使用。该核心提供蛋白质组学服务，包括二维凝胶、荧光二维差异凝胶电泳法，用于同时量化处理和未处理的组织或细胞之间数百种单个蛋白质的差异，并通过蛋白酶消化点的质谱分析来鉴定这些蛋白质。此外，该设施的核心提供了一个独特的、不断增长的100多个针对不同异源代谢酶及其转运体的单抗和多克隆抗体库的使用途径和经验。

Description (provided by applicant): The Proteomics Facility Core is designed to develop, adopt, and maintain state-of-the-art expertise and equipment for studies in proteomics, and to provide unique antibodies against xenobiotic metabolizing enzymes and their transporters for use by Center members. The core provides proteomic services including 2-D gels, fluorescent 2-D-difference gel electrophoresis for simultaneous quantification of the differences between treated and untreated tissues or cells in hundreds of individual proteins, and identification of these proteins by mass spectrometry of protease digested spots. In addition, the facility core provides access to and experience in the use of a unique and growing library of over 100 monoclonal and polyclonal antibodies specific for different xenobiotic metabolizing enzymes and their transporters.