Identification and characterization of novel PI3-kinase signal transducing elements in platelets

血小板中新型 PI3 激酶信号转导元件的鉴定和表征

• 批准号: nhmrc : 236905
• 负责人: Karen Anderson
• 金额: $ 30.51万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/identification-characterization-novel-elements-platelets/100672
• 关键词: Identification; characterization; novel; PI3; kinase

Platelets play an important role in blood clotting and blood vessel repair. Upon vessel injury, platelets rapidly adhere to the site of damage where they undergo dramatic shape change to spread over the site of injury. Activation and regulation of these processes relies on a complex network of signal transduction processes, involving the integration of multiple receptors and pathways. One pathway demonstrated to play a role in regulating platelet responses is the enzyme phosphatidylinositol 3-kinase (PI3-kinase) and its lipid products PtdIns(3,4,5)P3 and PtdIns(3,4)P2. However, very little is known about exactly how PI3-kinase and its products regulate the platelet responses. Our research studies aim to gain a deeper understanding into the molecular mechanisms of PI3-kinase signal transduction in platelets, through the identification and characterization of novel platelet proteins that bind to PI3-kinase lipid products, and to define what role these proteins play in platelet PI3-kinase dependent responses.

血小板在凝血和血管修复中起重要作用。在血管损伤时，血小板迅速粘附到损伤部位，在那里它们经历显著的形状变化以在损伤部位上扩散。这些过程的激活和调节依赖于复杂的信号转导过程网络，涉及多种受体和途径的整合。已证明在调节血小板应答中起作用的一种途径是磷脂酰肌醇3-激酶（PI 3-激酶）及其脂质产物PtdIns（3，4，5）P3和PtdIns（3，4）P2。然而，关于PI 3-激酶及其产物如何调节血小板反应的确切信息知之甚少。我们的研究旨在通过鉴定和表征与PI 3-激酶脂质产物结合的新型血小板蛋白质，并确定这些蛋白质在血小板PI 3-激酶依赖性反应中发挥的作用，从而更深入地了解血小板中PI 3-激酶信号转导的分子机制。