METHODS FOR CHARACTERIZING SUBSETS OF AMNIOTIC FLUID LEUKOCYTES

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our aim is to develop a fluorescence in situ hybridization (FISH) method for a rhesus Y-chromosome-specific gene in order to quantitate the percentage of fetal leukocytes in amniotic fluid and in tissues from pregnancies bearing a male fetus.