IMPLANTATION OF ESC-DERIVED SEROTONIN NEURONS INTO MACAQUE BRAIN

将 ESC 衍生的血清素神经元植入猕猴大脑中

• 批准号: 7958540
• 负责人: CYNTHIA Louise BETHEA
• 金额: $ 5.02万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-04 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958540
• 关键词: Brain; Computer Retrieval of Information on Scientific Projects Database; ES Cell Line; Funding; Grant; Green Fluorescent Proteins; Harvest; Hypothalamic structure; Implant; Institution; Lasers; Macaca; Macaca mulatta; Neurons; Primates; Reporting; Research; Research Personnel; Resources; Serotonin; Source; United States National Institutes of Health; embryonic stem cell; implantation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We reported previously that the rhesus embryonic stem cell line 366.4 could be induced to differentiate into cultures that were largely serotonin neurons. We proposed to harvest serotonergic neurospheres derived from 366.4 rhesus embryonic stem cells, which were transfected with green fluorescent protein (GFP). Then, the neurospheres were to be implanted bilaterally into the hypothalamus. After periods of 1-4 months, the brain was to be removed for neuroanatomical examination and laser capture of implanted neurons.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们以前报道过，恒河猴胚胎干细胞系366.4可以被诱导分化为主要是5-羟色胺神经元的培养物。我们建议从366.4恒河猴胚胎干细胞中收获多巴胺能神经球，这些细胞用绿色荧光蛋白（GFP）转染。然后，将神经球植入双侧下丘脑。1-4个月后，将脑取出进行神经解剖学检查和植入神经元的激光捕获。