IMAGING AND HISTOLOGY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 荧光成像核心为研究人员提供了可分析的细胞和组织图像。设备包括尼康E-800荧光显微镜、高分辨率视频和计算机驱动的图像捕获和分析系统。此外，BioRad Radiance 2000共聚焦显微镜和成像系统可用于确定特定蛋白质在组织切片或培养细胞中的亚细胞定位和分布。NIH成像、Image J、Image Pro Plus V6、LaserSharp和Photoshop软件均可使用。 我们提供组织标本的常规固定、包埋、切片和染色。该实验室设备齐全，可制备用于酶组织化学、免疫组织化学和免疫荧光的石蜡或冰冻包埋组织。我们的组织处理和组织学程序旨在优化组织固定，优化抗原和细胞细节的保存，并为组织切片定位，以实现最佳结构分析。组织学实验室维护用于切割石蜡包埋组织的Shandon Finesse 385和RMC MT-920显微切割机、Shandon Citadel 2000组织处理机、Shandon Histocentre包埋站、Shandon Veristain 24-4自动染色机和Shandon Cryotome冰冻组织切割机(人体和非人体组织各一台)。实验室也有可用的幻灯片、封面、安装介质、水浴和各种其他组织学工具。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Fluorescence Imaging Core provides researchers with analyzable images of cells and tissues. Equipment includes a Nikon E-800 fluorescent microscope, high resolution video and a computer- driven image capture and analysis system. Additionally, a BioRad Radiance 2000 confocal microscope and imaging system can be utilized for determining the subcellular localization and distribution of specific proteins in tissue slices or cultured cells. NIH Imaging, Image J, Image Pro Plus v6, LaserSharp and Photoshop software is available. We offer routine fixation, embedding, sectioning and staining of tissue specimens. The laboratory is fully equipped to prepare paraffin or frozen embedded tissue for enzyme histochemistry, immunohistochemistry and immunofluorescence. Our tissue processing and histology procedures are designed to optimize tissue fixation, optimize preservation of antigens and cellular detail and orient tissue for sectioning for the best structural analysis. The Histology laboratory maintains a Shandon Finesse 385 and RMC MT-920 microtomes for cutting paraffin embedded tissue, a Shandon Citadel 2000 tissue processor, a Shandon Histocentre embedding station, a Shandon Veristain 24-4 automated stainer and Shandon Cryotome microtomes for frozen tissues( one each for human and non human tissue). The lab also has available slides, coverslips, mounting media, waterbaths and a variety of other histology tools.