PROTEIN INTERACTION SCREENING IN YEAST

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 酵母资源中心（YRC）的使命是建立一个常规和全面的方法来研究真核生物的生物过程控制。该中心正在整合一套最先进的分析技术，从而系统地利用基因组序列信息及其在理解正常和异常细胞过程中的应用。最初，该中心的重点是一个模型的过程中，遗传特征良好的物种，其完整的基因组序列是可用的，酿酒酵母。合作已经建立，以扩大应用的综合方法，以其他细胞过程中的模式真核生物，S。啤酒。重要的是，已经做出了正在研究的过程的选择，部分原因是已经确定了已知在某些人类疾病中改变的调节系统中的保守组分。我们希望，随着人类基因组序列的日益完整，该中心的专业知识将非常适合于扩大对编码产物功能的了解。 在YRC和NFCR之间的这个合作项目中，我们将开发用于分析酵母蛋白质-蛋白质相互作用的改进方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The mission of the Yeast Resource Center (YRC) is to establish a routine and comprehensive approach to the study of the control of biological processes in eukaryotic organisms. The Center is integrating a set of state-of-the-art analytical technologies resulting in a systematic exploitation of genome sequence information and its application to understanding normal and abnormal cellular processes. Initially, the Center is focusing on a model process in genetically well-characterized species whose complete genome sequence is available, Saccharomyces cerevisiae. Collaborations are already being established to extend the application of the comprehensive approach to other cellular processes in the model eukaryotic organism, S. cerevisiae. Of importance, the choice of processes being studied has been made, in part, because of already identified conserved components in regulatory systems known to be altered in certain human diseases. We expect that as the sequence of the human genome becomes more complete the expertise of the Center will be well suited to expanding the knowledge of the function of the encoded products. In this Collaborative Project between the YRC and the NFCR, we will develop improved methods for analyzing yeast protein-protein interactions.