MOLECULAR MECHANISMS OF DNA PROTECTION IN SPORES OF BACILLUS SPECIES

芽孢杆菌孢子 DNA 保护的分子机制

• 批准号: 7722066
• 负责人: marek Jedrzejas
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722066
• 关键词: Acids; Bacillus (bacterium); Biochemical; Complex; Computer Retrieval of Information on Scientific Projects Database; Crystallography; DNA; DNA-Binding Proteins; Endopeptidases; Funding; Germination; Goals; Grant; Institution; Lead; Molecular; Peptide Hydrolases; Property; Proteins; Reproduction spores; Research; Research Personnel; Resources; Source; United States National Institutes of Health; novel; protein degradation; success; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objectives of the research proposed in this application are to investigate the mechanisms whereby a group of novel DNA-binding proteins, termed alpha/beta-type small, acid-soluble proteins (SASP) protect DNA in dormant spores of Bacillus species against a variety of damaging agents, and how these proteins are subsequently removed during spore outgrowth. The studies will involve biochemical and biophysical elucidation of the properties of the alpha/alpha-type SASP and the complexes of these proteins with DNA, as well as the characterization of the spore protease, termed Gpr, that initiates SASP degradation during spore outgrowth. A major goal of the proposed research is the characterization of all of these components including elucidation of their three-dimensional structures by x-ray crystallography. Success in achieving this goal will lead to an understanding at the molecular level of the mechanisms of DNA protection in dormant spores, as well the mechanisms responsible for the loss of this DNA protection during spore germination and outgrowth.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本申请中提出的研究的目的是调查一组新的DNA结合蛋白，称为α/β型小的酸溶性蛋白（SASP）保护芽孢杆菌属物种的休眠孢子中的DNA免受各种破坏剂的机制，以及这些蛋白质如何随后在孢子生长过程中被去除。这些研究将涉及α/α型SASP和这些蛋白质与DNA的复合物的特性的生物化学和生物物理学阐明，以及孢子蛋白酶的表征，称为Gpr，其在孢子生长期间启动SASP降解。拟议研究的一个主要目标是所有这些组件的表征，包括通过X射线晶体学阐明其三维结构。成功实现这一目标将导致在分子水平上理解休眠孢子中DNA保护的机制，以及孢子萌发和生长过程中DNA保护丧失的机制。