SERVICE

服务

• 批准号: 7724563
• 负责人: Milos Novotny
• 金额: $ 4万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724563
• 关键词: Cleaved cell; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Methods; Monosaccharides; Peptide Mapping; Peptides; Polysaccharides; Post-Translational Protein Processing; Protein Fingerprints; Proteins; Proteomics; Research; Research Personnel; Resources; Services; Source; Standards of Weights and Measures; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; glycosylation; liquid chromatography mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed Center will provide access to its existing methods as well as newly developed methods through a service component. The analytical services it will provide include a comprehensive set of methods for the analysis of monosaccharides using CE, polysaccharides cleaved from proteins using MS, and glycosylation of tryptic peptides using LC/MS methods. The services offered will also include standard proteomic analyses such as 2D gel electrophoresis, peptide fingerprinting for protein identification, and the analysis of post-translational protein modifications other than glycosylation.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 拟议的中心将通过一个服务部分提供对其现有方法以及新开发方法的使用。它将提供的分析服务包括一整套使用CE分析单糖的方法，使用MS从蛋白质中切割出的多糖，以及使用LC/MS方法对胰蛋白酶多肽进行糖基化的方法。提供的服务还将包括标准蛋白质组学分析，如2D凝胶电泳法、用于蛋白质鉴定的多肽指纹图谱，以及分析糖基化以外的翻译后蛋白质修饰。