DIFFUSION OF FLUORESCENTLY LABELED ENZYMES IN POLYMER FILMS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 罗丹明标记的醇脱氢酶被纳入伊士曼聚合物膜和FCS研究。 目的是确定蛋白质分子是否能够在聚合物基质中进行平移扩散。 FCS数据表明，没有显着的扩散，也发生了实质性的光漂白实验条件下。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rhodamine-labeled alcohol dehydrogenase was incorporated into an Eastman polymer film and studied by FCS. The goal was to ascertain if the protein molecules were able to undergo translational diffusion in the polymer matrix. The FCS data indicated no significant diffuion and also that subtantial photobleaching occured under the experimental conditions.