CYTOPLASMIC POLYHEDROSIS VIRUS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 呼肠孤病毒科中的病毒是能够在完整衣壳内进行转录和转录后加工的独特分子机器。细胞质型多角体病毒（CPV）属于呼肠孤病毒科（Reoviridae）胞质型多角体病毒属（cypovirus），是一种广泛分布的昆虫病原病毒。与其他呼肠孤病毒科病毒的多壳组织不同，CPV具有直径约600 º的单壳衣壳，包含10节段dsRNA基因组。 其相对简单的结构组织和无与伦比的衣壳稳定性使其成为研究内源RNA转录的结构基础和推动电子冷冻显微镜重建的分辨率极限的理想系统。本项目旨在利用单粒子冷冻电镜技术和二十面体重建方法对4-5埃分辨率进行研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Viruses in the family Reoviridae are unique molecular machines capable of transcription and post-transcription processing within the intact capsid. Cytoplasmic polyhedrosis virus (CPV), a widespread insect pathogen, belongs to the cypovirus genus in the Reoviridae family. Unlike the multiple-shelled organization of other Reoviridae viruses, CPV has a single-shelled capsid about 600 ¿ in diameter enclosing a 10-segmented dsRNA genome. Its relatively simple structural organization and unparalleled capsid stability make it an ideal system both for studying the structural basis of the endogenous RNA transcription and for pushing the resolution limit of electron cryomicroscopy reconstruction. This project is aimed at 4-5 Angstrom resolution study by single particle cryoEM with icosahedral reconstruction method.