CYTOPLASMIC POLYHEDROSIS VIRUS

胞质多角体病毒

• 批准号: 7721129
• 负责人: ZHONG ZHOU
• 金额: $ 1.62万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721129
• 关键词: Caliber; Capsid; Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; Cypovirus; Cytoplasmic Polyhedrosis Viruses; Double-Stranded RNA; Family; Funding; Genetic Transcription; Genome; Grant; Insecta; Institution; Methods; Molecular Machines; RNA; Reoviridae; Research; Research Personnel; Resolution; Resources; Source; System; Transcription Process; United States National Institutes of Health; Virus; base; particle; pathogen; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Viruses in the family Reoviridae are unique molecular machines capable of transcription and post-transcription processing within the intact capsid. Cytoplasmic polyhedrosis virus (CPV), a widespread insect pathogen, belongs to the cypovirus genus in the Reoviridae family. Unlike the multiple-shelled organization of other Reoviridae viruses, CPV has a single-shelled capsid about 600 ¿ in diameter enclosing a 10-segmented dsRNA genome. Its relatively simple structural organization and unparalleled capsid stability make it an ideal system both for studying the structural basis of the endogenous RNA transcription and for pushing the resolution limit of electron cryomicroscopy reconstruction. This project is aimed at 4-5 Angstrom resolution study by single particle cryoEM with icosahedral reconstruction method.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 呼肠孤病毒科的病毒是一种独特的分子机器，能够在完整的衣壳内转录和转录后处理。细胞质多角体病毒(CPV)属于呼肠孤病毒科的轮状病毒属，是一种分布广泛的昆虫病原体。与其他呼肠孤病毒科病毒的多壳组织不同，CPV有一个直径约600？的单壳衣壳，包裹着一个10个节段的dsRNA基因组。其相对简单的结构组织和无与伦比的衣壳稳定性使其成为研究内源RNA转录的结构基础和推动电子冷冻显微镜重建分辨率极限的理想系统。本项目是基于二十面体重建方法的单粒子低温电子显微镜4-5埃分辨率研究。