DISTRIBUTION AND CLEARANCE OF NASAL VACCINES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们工作的目标是更好地了解以喷雾剂(液体或干粉)形式鼻腔注射的疫苗的沉积位置及其清除率。将对与鼻用疫苗免疫原性增强或降低有关的各种疫苗配方(液体和干粉)进行评估，以确定疫苗沉积的位置和清除率是否与疫苗的效力/免疫原性有关。 成像研究对这项工作至关重要。根据诱导的抗原特异性免疫反应的大小，大型动物(豚鼠、兔子、非人灵长类动物)的鼻免疫不如小鼠鼻免疫有效。我们的假设是，由于较大动物的疫苗接种无效，对较大动物的鼻腔免疫并不像在老鼠身上观察到的那样有效。 我们希望确定鼻腔粘膜表面的鼻用疫苗配方，并确定疫苗随时间的移动(粘液纤毛清除)。此外，我们希望确定改变疫苗免疫原性的疫苗配方是否会影响其在体内的分布和清除。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of our work is to better understand the site of deposition of vaccines administered nasally as a spray (liquid or dry powder) and their rate of clearance. Various vaccine formulations (both liquid and dry powder) that are associated with increased or decreased immunogenicity of the nasally administered vaccine will be evaluated to determine if the site of vaccine deposition and the rate of clearance are associated with the efficacy/immunogenicity of the vaccine. Imaging studies are crucial for this work. Nasal immunization of large animals (guinea pigs, rabbits, non-human primates) is not as effective as nasal immunization of mice based on the magnitude of the antigen-specific immune response induced. Our hypothesis is that nasal immunization of larger animals is not as effective as observed in mice due to ineffective vaccine delivery of larger animals. We would like to identify the nasal vaccine formula on the nasal mucosal surfaces and determine the movement of the vaccine over time (mucociliary clearance). Additionally, we would like to determine if vaccine formulations that alter the immunogenicity of the vaccine affect its distribution and clearance in vivo.