MURINE PELVIC ANATOMY

鼠盆腔解剖学

• 批准号: 7726198
• 负责人: RONALD W WOOD
• 金额: $ 0.65万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7726198
• 关键词: Address; Anatomy; Animals; Automobile Driving; Biochemistry; Biophysics; Clinical; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; Disease; Female; Funding; Genitourinary system; Grant; Hearing; Image; Institution; Investigation; Label; Lower urinary tract; Microscopy; Missouri; Modeling; Mus; Mutation; Oregon; Organ; Pathologist; Pathology; Pelvis; Ptosis; Publishing; Purpose; Research; Research Personnel; Resolution; Resources; Source; Specimen; Students; Talents; Thinking; Tissue Sample; United States National Institutes of Health; Work; experience; interest; male; medical specialties; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DATA OF VISIBLE MOUSE SHARED WITH INVESTIGATOR We are interested in disorders of the genitourinary system, and have commenced doing MR microscopy of our pathology specimens. We are finding very little detailed study of normal anatomy, and we think it makes sense to use a common dataset in this context. The Duke "visible mouse" reference is very appealing to us. Hence we are turning to the Duke CIVM to request access to male and female datasets, as the published images of C57Bl6 mice are clearly exquisite. C57Bl6/J, FVB, CRL-CD1 strains are commonly used and would be of interest to us. Our focus has been detailed high-resolution MRmicroscopy of tissue samples prior to pathology studies. We have not done whole animal or even whole pelvis normal animals at this point. We have encountered what appears to be a dominant mutation in one of our colonies resulting in pelvic organ prolapse. We are attempting to determine if this serendiptous finding warrants further study, and we need examples of normal murine anatomy for comparison purposes. We would attempt to generate labelled images of the pertinent pelvic anatomy. The prolapse model was detected in a thesis by Aimee Johnson, a doctoral student of Dr. Jay Reeder in Pathology; both are my collaborators on multiple projects. My colleagues in the division of urogynecologic specialties heard of this model, they became very interested as prolapse is a significant clinical problem (Gunhilde Buchsbaum, Michael Flynn, Anna McNanly). We have been collaborating with Scott Kennedy in Biochemistry and Biophysics, our true MR microscopy talent. We are experienced ImageJ and Amira users.. Amira being our preferred tool once the data set undergoes initial manipulation. We have published some microCT work of the lower urinary tract with R. Ning. The control dataset is clearly of broader significance than what is driving this initial investigation, and will be of interest to a wider audience. We have collaborations in place with two veterinary pathologists, Dr. Raymond Baggs and Dr. Cynthia Besch-Williford (Oregon and Missouri, respectively) to whom we routinely turn for their special expertise. Interest in MR microscopy is growing at our institution, and the resource is currently limited. If demand increases or specific technical problems cannot be addressed, we may need to turn to CIVM for the conduct of imaging studies.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 与研究人员共享的可见小鼠数据 我们对泌尿生殖系统疾病感兴趣，并开始对我们的病理标本进行核磁共振显微镜检查。 我们发现对正常解剖学的详细研究很少，我们认为在这种情况下使用通用数据集是有意义的。 杜克大学的“可见鼠标”参考对我们来说非常有吸引力。 因此，我们向杜克大学 CIVM 请求访问雄性和雌性数据集，因为已发布的 C57Bl6 小鼠图像显然非常精美。 C57Bl6/J、FVB、CRL-CD1 菌株是常用的并且是我们感兴趣的。 我们的重点是在病理学研究之前对组织样本进行详细的高分辨率磁共振显微镜检查。目前我们还没有完成整个动物甚至整个骨盆正常动物。我们在我们的一个菌落中遇到了似乎是显性突变，导致盆腔器官脱垂。我们正试图确定这一偶然发现是否值得进一步研究，并且我们需要正常小鼠解剖学的例子来进行比较。 我们将尝试生成相关骨盆解剖结构的标记图像。 Jay Reeder博士的病理学博士生Aimee Johnson在一篇论文中发现了脱垂模型；两人都是我在多个项目上的合作者。 我泌尿妇科专业部门的同事听说了这个模型，他们变得非常感兴趣，因为脱垂是一个重要的临床问题（Gunhilde Buchsbaum，Michael 弗林，安娜·麦克南利）。 我们一直与 Scott Kennedy 在生物化学和生物物理学领域合作，他是我们真正的 MR 显微镜人才。 我们是经验丰富的 ImageJ 和 Amira 用户。一旦数据集经过初始操作，Amira 就会成为我们的首选工具。 我们与 R. Ning 发表了一些下尿路显微 CT 作品。 控制数据集显然比推动这项初步调查的数据具有更广泛的意义，并且会引起更广泛的受众的兴趣。 我们与两位兽医病理学家 Raymond Baggs 博士和 Cynthia Besch-Williford 博士（分别为俄勒冈州和密苏里州）建立了合作关系，我们经常向他们寻求特殊的帮助 专业知识。 我们机构对 MR 显微镜的兴趣与日俱增，但目前资源有限。如果需求增加或无法解决特定的技术问题，我们可能需要求助于 CIVM 进行成像 研究。