MURINE PELVIC ANATOMY

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DATA OF VISIBLE MOUSE SHARED WITH INVESTIGATOR We are interested in disorders of the genitourinary system, and have commenced doing MR microscopy of our pathology specimens. We are finding very little detailed study of normal anatomy, and we think it makes sense to use a common dataset in this context. The Duke "visible mouse" reference is very appealing to us. Hence we are turning to the Duke CIVM to request access to male and female datasets, as the published images of C57Bl6 mice are clearly exquisite. C57Bl6/J, FVB, CRL-CD1 strains are commonly used and would be of interest to us. Our focus has been detailed high-resolution MRmicroscopy of tissue samples prior to pathology studies. We have not done whole animal or even whole pelvis normal animals at this point. We have encountered what appears to be a dominant mutation in one of our colonies resulting in pelvic organ prolapse. We are attempting to determine if this serendiptous finding warrants further study, and we need examples of normal murine anatomy for comparison purposes. We would attempt to generate labelled images of the pertinent pelvic anatomy. The prolapse model was detected in a thesis by Aimee Johnson, a doctoral student of Dr. Jay Reeder in Pathology; both are my collaborators on multiple projects. My colleagues in the division of urogynecologic specialties heard of this model, they became very interested as prolapse is a significant clinical problem (Gunhilde Buchsbaum, Michael Flynn, Anna McNanly). We have been collaborating with Scott Kennedy in Biochemistry and Biophysics, our true MR microscopy talent. We are experienced ImageJ and Amira users.. Amira being our preferred tool once the data set undergoes initial manipulation. We have published some microCT work of the lower urinary tract with R. Ning. The control dataset is clearly of broader significance than what is driving this initial investigation, and will be of interest to a wider audience. We have collaborations in place with two veterinary pathologists, Dr. Raymond Baggs and Dr. Cynthia Besch-Williford (Oregon and Missouri, respectively) to whom we routinely turn for their special expertise. Interest in MR microscopy is growing at our institution, and the resource is currently limited. If demand increases or specific technical problems cannot be addressed, we may need to turn to CIVM for the conduct of imaging studies.