Iron Acquisition Mechanisms in Oligodendrocytes

据世界卫生组织称，缺铁是世界上最严重的营养失调症。铁对正常的神经功能至关重要，缺铁会导致认知和运动障碍，这种障碍会持续一生，而且往往是不可逆转的。许多与缺铁有关的神经系统问题可追溯到髓鞘发育不足。铁在髓鞘形成中的作用是在过去的20年里建立起来的，PI实验室的数据显示，少突胶质细胞比大脑中任何其他类型的细胞都更明显地染色铁。这些数据与参与髓鞘形成的相对高浓度的需要铁的酶一致。然而，在关于铁和少突胶质细胞功能的范式中，一个重要的遗漏，即少突胶质细胞如何获得铁，尚未确定。我们和其他人报道，尽管白质束中的铁含量相对较高，但在白质中没有可检测到的转移受体（传统的铁获取细胞机制）。即使当缺铁严重到导致髓鞘发育不足时，在白质中也检测不到传递受体的表达。我们提出了一个总体假设，即h -铁蛋白是少突胶质细胞的铁转运载体，而不是转铁蛋白。最近，信号蛋白家族的一员，t细胞免疫球蛋白粘蛋白结构域2 （Tim-2）被发现与h -铁蛋白结合。因此，这条研究路线的概念框架是Tim-2是铁蛋白结合蛋白，由少突胶质细胞选择性表达，是这些细胞获得产生和维持髓磷脂所需的铁的机制。这项研究的意义在于，我们发现了一种新的、受发育调节的、选择性表达的少突胶质细胞铁获取受体。由于Tim-2唯一已知的其他配体是Sema4A，一种在抗原提呈细胞和活化的Band T淋巴细胞上表达的蛋白质，因此本文提出的研究可以追求的一个潜在的重要未来方向将是探索免疫系统与少突胶质细胞之间通过Tim-2在少突胶质细胞上的表达可能影响脱髓鞘疾病的联系的可能性。该项目具有创新性，因为其目的是建立以下关于铁在eNS髓鞘形成中的作用的新数据：i) h -铁蛋白是少突胶质细胞获得铁的最终机制；ii)少突胶质细胞上的h -铁蛋白结合蛋白是Tim-2， iii) h -铁蛋白可以作为递送载体改善缺铁后的髓鞘形成。了解铁是如何被管理和传递到少突胶质细胞的，以及铁获取蛋白的表达时间，可以为治疗由铁缺乏、脱髓鞘疾病和成人（如多发性硬化症）的再髓鞘脱髓鞘疾病引起的发育性低髓鞘形成提供干预策略。

According to The World Health Organization, iron deficiency is the foremost nutritional disorder in the world. Iron is essential for normal neurological function and iron deficiency results in cognitive and motor impairments that can last throughout life, and are often irreversible. Many of the neurological problems associated with iron deficiency can be traced to hypomyelination. The role of iron in myelination was established over the past 20 years, by data from the PI's laboratory revealing that oligodendrocytes stain more prominently than any other cell type in the brain for iron. These data were consistent with the relatively high concentration of iron-requiring enzymes involved in myelination. However, a significant omission in the paradigm regarding iron and oligodendrocyte function, how oligodendrocytes acquire iron, has not been identified. We and others reported that despite the relatively high levels of iron in white matter tracts, there was no detectable transferring receptor (the traditional cellular mechanism for iron acquisition) in white matter. Even when iron deficiency is so severe as to cause hypomyelination, transferring receptor expression in white matter is not detectable. We propose the overall hypothesis that H-ferritin is the iron delivery vehicle for oligodendrocytes instead of transferrin. Recently, a member of the semaphorin family, T-cell immunoglobulin mucin domain 2 (Tim-2) was discovered to bind H-ferritin. Thus, the conceptual framework for this line of research is that Tim-2 is the ferritin binding protein that is selectively expressed by oligodendrocytes and is the mechanism by which these cells obtain the iron that is required to produce and sustain myelin. The significance of the proposed research is that we have found a novel, developmentally regulated, selectively expressed, receptor for iron acquisition on oligodendrocytes. Because the only other known ligand for Tim-2 is Sema4A, a protein expressed on antigen presenting cells and activated Band T lymphocytes, a potentially significant future direction that can be pursued following the studies proposed herein will be to explore the possibility of a connection between the immune system and oligodendrocytes via Tim-2 expression on oligodendrocytes that could affect demyelinating disorders. The project is innovative because the aims are designed to establish the following new data on the role of iron in myelination of eNS: i) H-ferritin is the definitive mechanism by which oligodendrocytes acquire iron; ii) the H-ferritin binding protein on oligodendrocytes is Tim-2, iii) that H-ferritin protein can be used as a delivery vehicle to improve myelination following iron deficiency. The knowledge of how iron is managed and delivered to oligodendrocytes as well as the timing of expression of iron acquisition proteins can be expected to inform intervention strategies for the treatment of developmental hypomyelination resulting from iron deficiency and demyelinating disorders and remyelination attempts in the adult such as Multiple Sclerosis.