Structure function analysis of the Dengue virus capsid protein.

登革热病毒衣壳蛋白的结构功能分析。

• 批准号: G0401586/1
• 负责人: Andrew Davidson
• 金额: $ 21.66万
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0401586%2F1
• 关键词: Structure; function; analysis; Dengue; virus

Dengue is the most important mosquito-borne viral disease of humans with 50-100 million people infected annually worldwide. Dengue virus infection usually results in dengue fever, a mild ?flu? like illness. However, in some cases, patients may develop severe, potentially fatal complications known as dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS). Despite intensive research over recent years, the disease processes leading to DHF/DSS are still poorly understood and there is neither a safe and effective vaccine nor suitable anti-viral treatments to control dengue disease.This project aims to study the function/s of the dengue virus capsid protein in the virus lifecycle and its possible contribution to disease processes. Dengue virus particles consist of multiple copies of only three proteins. A lipid membrane containing two surface proteins surrounds an inner core consisting of the capsid protein and the viral RNA genome. Aside from its role in the formation of the virus particle, the capsid protein has also been found in the host cell nucleus where it has the potential to disturb host cell processes. We will use recent information about the three dimensional structure of the capsid protein, in combination with genetic engineering of the viral genome, to identify regions of the capsid protein essential to the virus lifecycle. By determining how these regions of the C protein function in the virus lifecycle, we will increase our understanding of dengue virus replication and disease, and provide knowledge that can be used to develop novel dengue vaccines and antiviral compounds. The results of this investigation will also be relevant to related flaviviruses such as West Nile virus, Yellow fever virus and tick-borne and Japanese encephalitis viruses which cause public health problems worldwide.Staff at the University of Bristol are actively encouraged to communicate the results of their research to the general public. The results of University research is disseminated to the general public through articles in the publication ?re:search?, widely distributed in the community and by the organisation of research seminars open to the general public.

登革热是人类最重要的蚊媒病毒性疾病，全世界每年有5000万至1亿人感染。登革热病毒感染通常导致登革热，一个温和的？流感？就像生病一样。然而，在某些情况下，患者可能会出现严重的，可能致命的并发症，称为登革出血热/登革休克综合征（DHF/DSS）。本研究旨在探讨登革病毒衣壳蛋白在登革病毒生命周期中的功能及其在疾病过程中的作用。登革病毒颗粒仅由三种蛋白质的多个拷贝组成。含有两种表面蛋白的脂质膜包围由衣壳蛋白和病毒RNA基因组组成的内核。除了其在病毒颗粒形成中的作用外，还在宿主细胞核中发现了衣壳蛋白，在那里它具有干扰宿主细胞过程的潜力。我们将利用有关衣壳蛋白三维结构的最新信息，结合病毒基因组的基因工程，来识别对病毒生命周期至关重要的衣壳蛋白区域。通过确定C蛋白的这些区域在病毒生命周期中的功能，我们将增加对登革热病毒复制和疾病的了解，并提供可用于开发新型登革热疫苗和抗病毒化合物的知识。这项调查的结果也将与相关的黄病毒相关，例如西尼罗河病毒、黄热病病毒以及蜱传病毒和日本脑炎病毒，这些病毒会在全球范围内引起公共卫生问题。我们积极鼓励布里斯托大学的工作人员向公众传播他们的研究结果。大学的研究成果通过出版物中的文章传播给公众？re：search？，在社区广泛分发，并组织向公众开放的研究研讨会。