Southern Research Specialized Biocontainment Screening Center (SRSBSC)

南方研究专业生物防护筛选中心 (SRSBSC)

• 批准号: 7938938
• 负责人: E. Lucile White
• 金额: $ 257.38万
• 依托单位: SOUTHERN RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7938938
• 关键词: Southern; Research; Specialized; Biocontainment; Screening

Southern Research Institute (Southern Research) offers the Southern Research Specialized Biocontainment Screening Center (SRSBSC).for participation in the Molecular Libraries Program. The SRSBSC will provide a platform for assays requiring Biosafety Levels 2 and 3 (BSL2/3) for HTS. The current state-of-the-art in HTS in virology is limited by existing technology, which is primarily focused on screening of viral protein/enzyme-based targets or measuring the cytopathic effect of some viruses. These assay formats are highly amenable to HTS. However, a review of the literature reveals outstanding innovation by numerous laboratories in assays that can be adapted to an HTS platform, but which are not amenable to HTS because of the current state-of-the art of the instrumentation and reporter probes used in these analyses. Hence we will provide the opportunity to include these other lower throughput screening methods in the Network. The Center will have five functional Cores: (1) Assay Development/Adaptation/Implementation; (2) HTS; (3) Informatics, (4) Administrative and Management and (5) Research. The overarching objective is to provide 5 assays per year that identify molecular probes for protein function and pathways involved in virus-cell interactions, which will lead to hit identification and characterization. Further, we will work with the Specialized Chemistry and/or Comprehensive Screening Centers to develop a strategic plan for hit-to-probe optimization. It is our goal to meet the following milestones in Year 1: (1) Provide oversight and logistics required to move 5 assays forward through different Cores, (2) Enhance performance of dual reporter assays to accelerate 2 screening platforms in the BSL3: Reader and microscope technologies, (3) Provide technological solutions tooptimize throughput and robustness of 5 assays for automation in BSL2/3 containment, (4) Develop 5 counter screens unique to 'the 5 screening campaigns with Assay Provider, (5) Provide screening of 5 assays of 300,000 compounds, dose response of hits and curate associated data for public dissemination. In all of these activities, we will seek to integrate our Center with the MLPCN Research Network to promote collaborations,integrative solutions and sharing of data to achieve the goals of the Molecular Libraries initiative.

南方研究所（南方研究）提供南方研究专业生物防护筛选中心（SRSBSC）。SRSBSC将为HTS要求生物安全水平2和3（BSL 2/3）的试验提供平台。目前病毒学HTS的最新技术水平受到现有技术的限制，该技术主要集中在筛选病毒蛋白/酶靶点或测量某些病毒的细胞病变效应。这些测定形式非常适合HTS。然而，文献综述揭示了许多实验室在可以适应HTS平台的测定中的杰出创新，但由于这些分析中使用的仪器和报告探针的当前技术水平，这些实验室不适合HTS。因此，我们将提供机会，将这些其他低通量筛选方法纳入网络。 该中心将有五个功能核心：（1）检测开发/适应/实施;（2）HTS;（3）信息学;（4）行政和管理;（5）研究。总体目标是每年提供5种检测方法，用于识别病毒-细胞相互作用中涉及的蛋白质功能和途径的分子探针，这将导致命中识别和表征。此外，我们将与专业化学和/或综合筛查中心合作，制定一项针对命中探针优化的战略计划。我们的目标是在第1年实现以下里程碑：（1）提供通过不同核心推进5项检测所需的监督和后勤保障，（2）提高双报告基因检测的性能，以加速BSL 3中的2个筛选平台：阅读器和显微镜技术，（3）提供技术解决方案，以优化BSL 2/3遏制中自动化的5种测定的通量和稳健性，（4）与检测供应商一起开发5个筛选活动特有的5个计数器筛选，（5）提供300，000种化合物的5个检测的筛选，命中的剂量响应和策划相关数据以供公众传播。在所有这些活动中，我们将寻求将我们的中心与MLPCN研究网络整合，以促进合作，综合解决方案和数据共享，以实现分子图书馆计划的目标。