La Jolla Interdisciplinary Neuroscience Center Cores - Pro Core Salk

该奖项的一个主要目标是整合La Jolla神经生物学的蛋白质组学工作 伯纳姆医学研究所现有的蛋白质组学中心。这将是 涉及购买额外的仪器(在第一年购买LTQ离子陷阱)，以及雇用 额外的人员。通过融合神经科学蛋白质组学共享的资源 当前中心(包括伯纳姆癌症中心的蛋白质组学设施以及 NTCNP蛋白质分解途径中心)，我们将能够利用相当大的 现有中心的仪器和专业知识，以造福神经科学界。这个 目前的拨款申请将促进神经科学家平等使用这些设施，而不是 目前可以进入，因为设施在癌症中心。神经科学的整合 进入现有蛋白质组学中心的蛋白质组学工作将为 神经科学蛋白质组学研究。除了伯纳姆研究所现有的设备外， 上面列出的医学研究(BIMR)，是在Jeff Smith博士的指导下，卫星设施 在索尔克研究所(在沃尔夫冈·费舍尔的指导下)和斯克里普斯也有 研究所(TSRI)，在著名蛋白质组学权威机构John Yates，III的指导下 例如，当需要更大的蛋白质或非常精确的质量测量时， 最近在TSRI安装的Finnigan LTQ/FT仪器将向神经科学家提供。 TSRI的一名技术人员将被指定专门与 神经科学界在这方面的研究。此外，在索尔克的卫星蛋白质组学设施 布鲁克Ultraflex质谱仪有一个用于对SELDI上的多肽进行测序的界面 芯片最初的特点是位于各个实验室的较小的先锋基因系统 BIMR、Salk或TSRI。因此，BIMR(枢纽设施)和Salk和TSRI(枢纽设施)的三个设施 将共同努力，使彼此的设备得到最大限度的利用。史密斯博士，耶茨博士， 费舍尔将每月开会讨论使用情况，确定项目的优先顺序，并确保神经科学家 进入。

A primary objective for this award is to integrate the proteomics efforts of the La Jolla Neurobiology community into the existing Proteomics Center at the Burnham Institute for Medical Research. This will involve the purchase of additional instrumentation (an LTQ Ion Trap in Year 1), and the hiring of additional personnel. By amalgamating the neuroscience Proteomics Shared Resource with that of the current Center (which includes the Proteomics Facility for the Burnham's Cancer Center as well as the NTCNP Center on Proteolytic Pathways), we will be able to capitalize on the considerable instrumentation and expertise of the existing Center for the benefit of the Neuroscience community. The current grant application will facilitate equal access to these facilities by Neuroscientists, who do not have access currently because the facility is in the Cancer Center. The integration of the Neuroscience proteomics effort into the existing Proteomics Center will provide a substantial jump-start for the Neuroscience proteomics effort. In addition to the current equipment at the Burnham Institute for Medical Research (BIMR) listed above, which is under the direction of Dr. Jeff Smith, satellite facilities are also available at The Salk Institute (under the direction of Wolfgang Fischer) and at The Scripps Research Institute (TSRI), under the direction of noted proteomics authority John Yates, III. For example, when required for larger proteins or for very precise measurements of mass, the recently-installed Finnigan LTQ/FT instrument at TSRI will be made available to neuroscientists. A technician at TSRI will be designated to specifically interact with and meet the needs of the Neuroscience Community in this regard. Additionally, at the satellite proteomics facility at The Salk Institute, The Bruker Ultraflex Mass Spectrometer has an interface for sequencing peptides on SELDI CHIPs initially characterized by smaller Chiphergen systems located in individual laboratories at the BIMR, Salk, or TSRI. Hence, the three facilities at BIMR (hub facility) and Salk and TSRI (hub facilities) will work together to allow each other's equipment to be used for maximal efficiency. Drs. Smith, Yates, and Fischer will meet monthly to discuss usage, prioritize projects, and insure that neuroscientists have access.