Experimental Component

该提案的主要主题是“肿瘤异质性对癌症进展的影响”。因此，实验部分旨在根据癌症进展的特征，如增殖、新陈代谢和运动性，量化癌细胞的异质性。我们将在几个Me扰动下，量化模型培养细胞系中的这种特征异质性，通过高含量自动显微镜和图像处理在单细胞水平上进行平均和分布测量。性状分布也将被分解为统计子群体，以揭示共同的趋势 在细胞系之间。几个体内肿瘤实验系统将补充体外测试的目的，以验证理论模型。我们已经组建了一支才华横溢的实验者团队，他们涵盖了这一部件所需的所有特定技能，正如他们杰出的出版记录所示。员工科学家将确保组件技术的连续性。

The overarching theme of this proposal is "Impact of Tumor Heterogeneity on Cancer Progression". The Experimental Component is therefore geared to quantifying cancer cell heterogeneity with respect to hallmark traits of cancer progression, such as proliferation, metabolism and motility. We will quantify this trait heterogeneity in model cultured cell lines, under several mE perturbation, as average and distribution measurements performed at the single-cell level by high-content automated microscopy and image processing. Trait distributions will also be resolved as statistical subpopulations, in order to uncover common trends between cell lines. Several In vivo tumor experimental systems will complement in vitro assays for the purpose of theoretical model validation. We have assembled a team of talented experimentalists that cover all specific skills needed for this Component, as shown by their distinguished publication records. Staff scientists will assure continuity of Component technology.