REDOX REGULATION OF HYPOXIA INDUCIBLE FACTOR (HIF)-1 IN CEREBRAL ISCHEMIA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 项目一：皮红莲施 描述 氧和葡萄糖供应的减少涉及许多病理情况，如中风，并导致缺血性损伤。糖、氧供应和代谢失衡导致氧化应激，改变细胞氧化还原(还原/氧化)环境。细胞氧化还原环境对细胞信号的调控和细胞的存活起着至关重要的作用。低氧诱导因子1是氧化还原调节的分子靶点之一，已被发现在脑缺血时细胞死亡和存活中起关键作用。我们正在进行的项目将利用北卡罗来纳大学脑中心现有的最先进技术，通过体外和体内模型研究缺血性中风的氧化还原变化。我们的方法包括核磁共振、电子顺磁共振(EPR)光谱和成像、双光子显微镜、荧光显微镜以及细胞和分子技术。我们的目标是发现潜在的有效和安全的中风治疗方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project 1: PI  Honglian Shi Description Reduced supply of oxygen and glucose involves in many pathological conditions such as stroke and contributes to ischemic injuries. Imbalanced glucose and oxygen supplies and metabolism induces oxidative stress and alters cellular redox (reduction/oxidation) environment. Cellular redox environment is critical for the regulation of cellular signaling and cell survival. One of the molecular targets of redox regulation ise hypoxia inducible factor 1, which has been found to play a critical role in cell death and survival in cerebral ischemia. Our ongoing project will utilize state of the art technology available in UNM BRaIN center to study redox changes in ischemic stroke with in vitro and in vivo models. Our approaches include MRI, electron paramagnetic resonance (EPR) spectroscopy and imaging, two-photon microscopy, fluorescence microscopy, and cellular and molecular techniques. Our goal is to discover potential effective and safe approaches for stroke treatment.