Multicolor Fluorescent Analysis in Zebrafish

斑马鱼的多色荧光分析

• 批准号: 7935634
• 负责人: ALEXANDER F SCHIER
• 金额: $ 24.4万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7935634
• 关键词: Anatomy; Animal Model; Biological Models; Biological Process; Biology; Cell Nucleus; Cell division; Cells; Chimeric Proteins; Chromatin; Color; Congenital Abnormality; Development; Disease; Effectiveness; Embryo; Genetic Recombination; Goals; Human; Image; Immunohistochemistry; Label; Larva; Lead; Life; Membrane; Methods; Mus; Nervous system structure; Neurons; Organ; Pattern; Physiological Processes; Proteins; Protocols documentation; Reagent; Reporter; Reporter Genes; Research; Resolution; Subcellular structure; Synapses; Technology; Testing; Time; Toxic effect; Work; Zebrafish; combinatorial; in vivo; innovation; insight; next generation; novel; public health relevance; tool; zebrafish development

DESCRIPTION (provided by applicant): The translucency of zebrafish embryos and larvae makes them ideally suited for the in vivo analysis of developmental and physiological processes. This study aims to develop tools for the multicolor fluorescent labeling of zebrafish cells. Previous studies have demonstrated that stochastic DNA recombination can lead to the expression of different combinations of fluorescent proteins in different cells. The combinatorial expression of fluorescent proteins endows cells with many colors, thereby providing a way to distinguish adjacent cells and visualize cellular interactions. We will develop tools that make this technology widely and easily applicable to the study of zebrafish development and anatomy (Aim 1) and generate next-generation reagents and protocols that allow the sophisticated application of this technology to various aspects of zebrafish biology (Aim 2). PUBLIC HEALTH RELEVANCE: Zebrafish is a vertebrate model organism that shares many biological processes with humans. Research in zebrafish has provided important insights into the causes of birth defects and disease. The proposed work is to enhance the utility of this model system by developing tools to label cells in multiple different colors and follow their development.

描述(申请人提供)：斑马鱼胚胎和幼虫的半透明使它们非常适合于体内发育和生理过程的分析。本研究旨在开发斑马鱼细胞的多色荧光标记工具。先前的研究表明，随机DNA重组可以导致不同细胞中不同组合的荧光蛋白的表达。荧光蛋白的组合表达赋予细胞许多颜色，从而提供了一种区分相邻细胞和可视化细胞相互作用的方法。我们将开发工具，使这项技术广泛和容易地应用于斑马鱼发育和解剖学的研究(目标1)，并产生下一代试剂和协议，允许将这项技术复杂地应用于斑马鱼生物学的各个方面(目标2)。 与公共卫生相关：斑马鱼是一种脊椎动物模式生物，与人类有许多共同的生物学过程。对斑马鱼的研究为研究出生缺陷和疾病的原因提供了重要的见解。建议的工作是通过开发工具来标记多种不同颜色的细胞并跟踪它们的发展，以增强该模型系统的实用性。