Core B Mouse Tumor Models and Mammalian Cell Culture

动物模型中心将通过为一代人提供服务， 转基因小鼠模型的维持和分析。此外，核心将提供组织 用于组织学和生物化学分析的样本，并将产生上皮细胞系和原代培养物 用于体外分析。核心组件高度集成， 所有四个研究项目提供细胞和组织进行分析，并为三个 四个新小鼠模型的生产和维护项目（项目1、3、4）， 将采用现有的小鼠模型，包括Nox-1敲除小鼠，这些小鼠已经产生 以前由项目4（兰贝斯）与核心B主任（马丁），和线粒体！ 硫氧还蛋白转基因小鼠（TRX 2）先前在埃默里大学与马丁博士， 和迪恩·琼斯博士（附合作信）。本项目中使用的其他现有菌株 包括但不限于Villin k-rasv 12 Tg、APC 1638和ARC min小鼠。核心设施还将 为项目4（松鸡）生产和表征新型转基因模型villin-MTH-1。这是特别 重要的是，因为这个实验室没有以前的动物模型经验，将无法产生和 在没有核心组件专业知识的情况下分析这些老鼠。核心将维护和基因型， 殖民地，并提供实时殖民地数据访问所有计划参与者通过一个商业上可用的 关系数据库（Scion，黄玉技术公司），该数据库目前由核心董事实验室使用。 除了活体动物模型外，核心还将向该计划提供转化细胞系 参与者进行生化分析。这将是至关重要的几个项目，并将允许强有力的 马马良模型和酵母系统之间的联系。通过提供这些服务，动物 模型核心将从科学的角度为项目的总体目标做出重大贡献。 perspective.此外，将这些服务纳入一个全面的核心组成部分， 通过整合工作，避免不必要的 实验性重复和利用核心人员的集体专门知识。

The Animal Models Core will assist the overall program project by providing service for the generation, maintenance, and analysis of genetically modified mouse models. In addition, the core will provide tissue samples for histological and biochemical analysis, and will generate epithelial cell lines and primary cultures from the animal models for in-vitro analysis. The core component is highly integrative, being utilized by all four of the research projects for provision of cells and tissues for analysis, and for three of the four projects for the production and maintenance of novel mouse models (Project 1, 3, 4), Several existing mouse models will be employed, including Nox-1 knockout mice, which have been generated previously by Project 4 (Lambeth) in conjunction with the Director of Core B (Martin), and mitochondria! thioredoxin transgenic mice (TRX2) previously generated here at Emory in collaboration between Dr. Martin, and Dr. Dean Jones (letter of collaboration attached). Additional existing strains to be utilized in this program include, but may not be limited to, Villin k-rasv12 Tg, APC 1638, and ARC min mice. The core facility will also produce and characterize a novel transgenic model, villin-MTH-1 for Project 4 (Grouse). This is especially significant, since this lab has no previous animal models experience, and would not be able to generate and analyze these mice without the expertise of the core component. The core will maintain and genotype the colony, and provide real-time colony data access to all program participants through a comercially available relational database (Scion, Topaz Technologies) which is currently in use by the Core Directors laboratory. In addition to the live animal models, the core will also provide transformed cell lines to the program participants for biochemical analysis. This will be critical to several of the projects, and will allow for strong linkage between the mamallian models and the yeast system. By provision of these services, the Animal Models Core will contribute significantly to the overall goals of the program project from a scientific perspective. In addition, the inclusion of these services into a comprehensive core component will provide additional benefit to the project as a whole, through consolidation of effort, avoidance of unnecessary experimental duplication and by drawing upon the collective expertise of the core personnel.