Core B Mouse Tumor Models and Mammalian Cell Culture

动物模型核心将通过为世代提供服务来协助整个项目项目， 转基因小鼠模型的维护和分析。此外，核心将提供组织 用于组织学和生化分析的样本，并将产生上皮细胞系和原代培养物 从动物模型中进行体外分析。核心组件是高度集成的，由 所有四个为分析提供细胞和组织的研究项目，以及三个 生产和维护新型鼠标的四个项目(项目1、3、4)，若干 将使用现有的小鼠模型，包括已经产生的Nox-1基因敲除小鼠 之前由项目4(Lambeth)与核心B主任(Martin)合作，以及线粒体！ 硫氧还蛋白转基因小鼠(TRX2)之前在埃默里大学与马丁博士、 和迪恩·琼斯博士(附上合作信函)。将在该计划中使用的其他现有菌株 包括但不限于Villin k-rasv12 Tg、APC 1638和ARC Min小鼠。核心设施还将 为项目4(松鸡)建立并鉴定了一种新的转基因模型--Villin-MTH-1。这是特别的 重要的是，因为这个实验室以前没有动物模型的经验，并且不能产生和 在没有核心组件专业知识的情况下分析这些鼠标。核心将保持并对 殖民地，并为所有计划参与者提供实时殖民地数据访问 核心导向器实验室目前正在使用的关系数据库(SCHON、TOPAZ Technologies)。 除了活体动物模型外，核心还将为该计划提供转化的细胞系 参与者进行生化分析。这将对几个项目至关重要，并将允许强大的 哺乳动物模型和酵母系统之间的联系。通过提供这些服务，动物 Models Core将从科学的角度为项目的总体目标做出重大贡献 透视。此外，将这些服务纳入一个全面的核心组成部分将提供 通过巩固努力，为整个项目带来额外的好处，避免不必要的 通过试验性复制和利用核心人员的集体专长。

The Animal Models Core will assist the overall program project by providing service for the generation, maintenance, and analysis of genetically modified mouse models. In addition, the core will provide tissue samples for histological and biochemical analysis, and will generate epithelial cell lines and primary cultures from the animal models for in-vitro analysis. The core component is highly integrative, being utilized by all four of the research projects for provision of cells and tissues for analysis, and for three of the four projects for the production and maintenance of novel mouse models (Project 1, 3, 4), Several existing mouse models will be employed, including Nox-1 knockout mice, which have been generated previously by Project 4 (Lambeth) in conjunction with the Director of Core B (Martin), and mitochondria! thioredoxin transgenic mice (TRX2) previously generated here at Emory in collaboration between Dr. Martin, and Dr. Dean Jones (letter of collaboration attached). Additional existing strains to be utilized in this program include, but may not be limited to, Villin k-rasv12 Tg, APC 1638, and ARC min mice. The core facility will also produce and characterize a novel transgenic model, villin-MTH-1 for Project 4 (Grouse). This is especially significant, since this lab has no previous animal models experience, and would not be able to generate and analyze these mice without the expertise of the core component. The core will maintain and genotype the colony, and provide real-time colony data access to all program participants through a comercially available relational database (Scion, Topaz Technologies) which is currently in use by the Core Directors laboratory. In addition to the live animal models, the core will also provide transformed cell lines to the program participants for biochemical analysis. This will be critical to several of the projects, and will allow for strong linkage between the mamallian models and the yeast system. By provision of these services, the Animal Models Core will contribute significantly to the overall goals of the program project from a scientific perspective. In addition, the inclusion of these services into a comprehensive core component will provide additional benefit to the project as a whole, through consolidation of effort, avoidance of unnecessary experimental duplication and by drawing upon the collective expertise of the core personnel.