Flow Cytometry & Cell Sorting

Hollings癌症中心（HCC）流式细胞术和细胞分选（FCCS）共享资源提供HCC 成员全面的分析流式细胞术和最先进的高速细胞分选服务， 成本有效的方式。在周道宏医学博士的领导下，FCCS配备了一台Becton- Dickinson FACSCalibur分析流式细胞仪、Becton-Dickinson FACSAria细胞分选仪和Dako VIoFlo高速细胞分选仪。该资源的工作人员在高速分拣方面具有相当的专业知识 包括干/祖细胞和表位特异性T细胞在内的罕见细胞群体的研究， 扩大现有技术的库，以满足内部创新研究基地的需要 HCC。 自2003年以来，HCC FCCS共享资源支持了123篇出版物的研究 由33名研究人员代表所有的HCC计划和八个部门在医科大学， 南卡罗来纳州。HCC FCCS共享资源在几个开创性的 这是HCC成员在此期间的发现。HCC FCCS的具体目标 共享资源将： 提供多参数和多激光测量，包括测量：细胞凋亡，细胞 增殖、细胞周期分布和免疫表型，以及检测细胞内 蛋白质、膜扰动和生物活性分子 基于细胞表面标记免疫染色和/或侧群提供高速细胞分选 染色和绿色荧光蛋白的表达 <$提供有关实验设计、样品制备、数据等方面的咨询和协助 分析，并为出版物和赠款申请准备高质量的数字，因为它们与 流式细胞术 提供使用常规流式细胞仪技术的培训，并提供更先进的培训， HCC成员关于新开发的流式细胞术检测的意见 识别和开发新的流式细胞术技术，以协助HCC的前沿研究

The Hollings Cancer Center (HCC) Flow Cytometry & Cell Sorting (FCCS) Shared Resource provides HCC members comprehensive analytic flow cytometry and state-of-the-art high speed cell sorting services in a cost effective manner. Under the directorship of Daohong Zhou, MD, the FCCS is equipped with a Becton- Dickinson FACSCalibur Analytical Flow Cytometer, a Becton-Dickinson FACSAria Cell Sorter and a Dako VIoFlo High-Speed Cell Sorter. The staff of the resource have considerable expertise in high-speed sorting of rare populations of cells including stem/progenitor cells and epitope-specific T cells and are continually expanding the repertoire of available techniques to meet the needs of the innovative research base within the HCC. Since 2003, the HCC FCCS Shared Resource has supported research that has resulted in 123 publications by 33 investigators representing all of the HCC programs and eight Departments at the Medical University of South Carolina. The HCC FCCS Shared Resource has played an essential role in several of the seminal discoveries made by members of the HCC during this time. The specific objectives of the HCC FCCS Shared Resource are to: ¿ Provide multi-parametric and multi-laser measurements including measurements of: apoptosis, cell proliferation, cell cycle distribution, and immunophenotyping, as well as detection of intracellular proteins, membrane perturbations, and bioactive molecules ¿ Provide high-speed cell sorting based on cell surface marker immunostaining and/or side-population staining, and expression of green fluorescent protein ¿ Provide consultation and assistance concerning experimental design, sample preparation, data analysis, and preparation of high quality figures for publications and grant applications as they relate to flow cytometry ¿ Provide training in the use of routine flow cytometric technology and offer more advanced training for HCC members concerning the newly developed flow cytometric assays ¿ Identify and develop new flow cytometric technologies to assist cutting-edge research in the HCC