IMAGE CORRELATION SPECTROSCOPY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们想用图像相关光谱的新方法来确定盖玻片上每平方微米荧光团的数量密度。我们的实验包括将DNA分子移植到盖玻片上。然后，含有合适分子的互补DNA链与玻璃上的DNA分子杂交。我们猜测嫁接的DNA分子的密度大约是每平方微米100个。用羧基荧光素作为荧光标记物标记DNA。我们想学习图像相关光谱技术，这将需要一个低背景荧光设置的资源与其倒置显微镜。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We would like to determine the number density of fluorophores per square micrometer on a cover slide using the novel approach of image correlation spectroscopy. Our experiments involve grafting DNA molecules on glass coverslips. Complementary DNA strands containing a fitting molecule are then hybridized to the DNA molecules on the glass. We guess that the density of grafted DNA molecules is about 100 per square micrometer. The DNA is labeled with carboxy-fluorescein as fluorescent marker. We would like to learn the technique of image correlation spectroscopy which would require a low-background fluorescence set up the Resource has with its inverted microscope.