THE EFFECT OF D-AMINO ACIDS ON THE FOLDING DYNAMICS OF TRP-CAGE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 氰基苯丙氨酸(PheCN)在蛋白质折叠动力学的红外光谱研究中得到了广泛的应用，因为它具有强的和光谱隔离的氰基振动。最近我们发现了PheCN，我们记录了该修饰氨基酸的可见吸收光谱和发射光谱，发现其具有良好的荧光性质。典型地，吸收在270 nm区域，发射最大值在310 nm左右。我们建议在蛋白质折叠研究中作为局部发色团，特别是在D-氨基酸对Trp-CAGE折叠动力学的影响的研究中。D-氨基酸通常用于蛋白质设计，以提高蛋白质的稳定性。然而，D-氨基酸在蛋白质折叠中的动力学作用尚未被研究。因此，这个项目的目标是使用一个小的折叠基序Trp-CAGE，来系统地研究单个D-氨基酸对蛋白质折叠动力学的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cyanophenylalanine (PheCN) has found many applications in infrared spectroscopic studies of protein folding dynamics due to the strong and spectrally isolated nitrile vibration. Recently we discovered that PheCN we recorded visible absorption and emission spectra and found good fluorescence property of this modified aminoacid. Typically, absorption is in the 270nm region and emission maximum around 310nm. We propose to use in protein folding studies as a local chromophor, especially in the study on the effect of D-amino acids on the folding dynamics of Trp-cage. D-amino acids are commonly used in protein design to enhance protein stabilities. However, the kinetic role of D-amino acids in protein folding has not been studied. Thus, the goal of this project is to use a small folding motif, Trp-cage, to systematically examine the effect of individual D-amino acids on the folding kinetics of the protein.