THE EFFECT OF D-AMINO ACIDS ON THE FOLDING DYNAMICS OF TRP-CAGE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 氰基苯丙氨酸（PheCN）由于其强烈的光谱孤立振动，在蛋白质折叠动力学的红外光谱研究中有着广泛的应用。最近我们发现了PheCN，我们记录了它的可见吸收光谱和发射光谱，发现它具有良好的荧光性质。 典型地，吸收在270 nm区域中并且发射最大值在310 nm附近。我们建议在蛋白质折叠的研究中作为一个本地的发色团，特别是在研究的D-氨基酸对色氨酸笼的折叠动力学的影响。D-氨基酸通常用于蛋白质设计以增强蛋白质稳定性。然而，D-氨基酸在蛋白质折叠中的动力学作用尚未研究。因此，本项目的目标是使用一个小的折叠基序，色氨酸笼，系统地研究个别D-氨基酸对蛋白质折叠动力学的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cyanophenylalanine (PheCN) has found many applications in infrared spectroscopic studies of protein folding dynamics due to the strong and spectrally isolated nitrile vibration. Recently we discovered that PheCN we recorded visible absorption and emission spectra and found good fluorescence property of this modified aminoacid. Typically, absorption is in the 270nm region and emission maximum around 310nm. We propose to use in protein folding studies as a local chromophor, especially in the study on the effect of D-amino acids on the folding dynamics of Trp-cage. D-amino acids are commonly used in protein design to enhance protein stabilities. However, the kinetic role of D-amino acids in protein folding has not been studied. Thus, the goal of this project is to use a small folding motif, Trp-cage, to systematically examine the effect of individual D-amino acids on the folding kinetics of the protein.