KINETIC STUDIES OF ENZYME MECHANISMS

酶机制的动力学研究

• 批准号: 7954605
• 负责人: WILLIAM Wallace CLELAND
• 金额: $ 0.1万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954605
• 关键词: Carbon Dioxide; Computer Retrieval of Information on Scientific Projects Database; Diazomethane; Enzymes; Funding; Grant; Institution; Kinetics; Research; Research Personnel; Resources; Source; United States National Institutes of Health; carbonic acid, monoanhydride with phosphoric acid, ion(2-); enzyme mechanism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to seek experimental evidence to support the hypothesis that some enzymes use carboxyphosphate as a CO2 source. Carboxyphosphate will be made synthetically and derivatized with diazomethane. The product will be characterized by 31P and 13C NMR.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目的是寻找实验证据来支持某些酶使用羧基磷酸作为CO2源的假设。 羧基磷酸盐将合成并用重氮甲烷衍生。 产物将通过31 P和13 C NMR表征。