ENTH AND BAR DOMAINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 ENTH和BAR结构域是模块化的脂质结合结构域，其在网格蛋白介导的内吞作用期间与膜变形有关。从我们的实验室以及从别人的结果表明，这些领域的膜表面的自缔合是必不可少的膜变形活动。为了阐明这些域诱导膜变形的机制，我们建议测量它们的自关联的程度在一个巨大的单层囊泡的时间依赖性的方式由N和B分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ENTH and BAR domains are modular lipid-binding domains that have been implicated in membrane deformation during clathrin-mediated endocytosis. Results from our laboratory as well as from others have indicated that self-association of these domains on the membrane surface is essential for their membrane-deforming activities. To elucidate the mechanisms by which these domains induce membrane deformation, we propose to measure the degree of their self-association on giant unilamellar vesicles in a time-dependent manner by N and B analysis.