ENTH AND BAR DOMAINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 TEN和BAR结构域是模块化的脂结合结构域，在细胞骨架蛋白介导的内吞作用中与膜的变形有关。我们实验室和其他人的结果表明，这些结构域在膜表面的自结合对于它们的膜变形活动是必不可少的。为了阐明这些结构域导致膜变形的机制，我们建议通过N和B分析以时间依赖的方式测量它们在巨大单层囊泡上的自结合程度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ENTH and BAR domains are modular lipid-binding domains that have been implicated in membrane deformation during clathrin-mediated endocytosis. Results from our laboratory as well as from others have indicated that self-association of these domains on the membrane surface is essential for their membrane-deforming activities. To elucidate the mechanisms by which these domains induce membrane deformation, we propose to measure the degree of their self-association on giant unilamellar vesicles in a time-dependent manner by N and B analysis.