STRUCTURAL STUDY OF PHI 29 PRNA

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 枯草芽孢杆菌噬菌体 ¿29 的前头 RNA (pRNA) 是基因组最左端的 174 个碱基转录物，是 ¿29 DNA 包装马达的重要组成部分。 一种新型 pRNA 多聚体与前体蛋白壳 (prohead) 的头尾连接器结合，随后 ATP 酶 gp16 与 pRNA 的结合构成了易位 19 kilobase 对 DNA-基因产物 3 复合物 (DNA-gp3) 的马达。 ATP水解驱动DNA-gp3包装，一个ATP用于包装DNA的两个碱基对。 ¿29 中 pRNA 的发现是病毒 DNA 包装机制研究的领先系统，具有普遍意义，因为常见机制可用于包装有尾噬菌体和某些动物病毒的双链 DNA。 pRNA 作为 DNA 包装中的瞬时结构成分和催化剂的作用扩展了 RNA 新结构和生化功能的进化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prohead RNA (pRNA) of the Bacillus subtilis bacteriophage ¿29, a 174-base transcript of the far left end of the genome, is an essential component of the ¿29 DNA packaging motor. A novel pRNA multimer binds to the head-tail connector of the precursor protein shell (prohead), and subsequent binding of the ATPase gp16 to pRNA constitutes the motor that translocates the 19 kilobase pair DNA-gene product 3 complex (DNA-gp3). ATP hydrolysis drives DNA-gp3 packaging, and one ATP is used to package two base pairs of DNA. The discovery of pRNA in ¿29, the leading system for mechanistic studies of viral DNA packaging, has general significance in that common mechanisms may be used in packaging double-stranded DNAs of the tailed bacteriophages and certain animal viruses. The role for pRNA as a transient structural component and catalyst in DNA packaging extends the evolvement of novel structural and biochemical functions for RNA.