QUESTION OR TRAINING REQUEST FOR THE YEAST RESOURCE CENTER

对酵母资源中心的问题或培训请求

• 批准号: 7957701
• 负责人: Timothy G Formosa
• 金额: $ 0.48万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957701
• 关键词: Biology; Computer Retrieval of Information on Scientific Projects Database; Funding; Fungal Genome; Grant; Hybrids; Institution; Phosphotyrosine; Proteins; Research; Research Personnel; Resources; Source; Training; United States National Institutes of Health; Yeasts; interest; research study; src Homology Region 2 Domain; yeast protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in studying interactions with a structural domain of S cerevisiae Spt6 (the only known SH2 domain in yeast). Spt6 has several structural domains, so it is not clear that previous experiments that used the whole Spt6 protein will be relevant. As yeast are not known to have phosphotyrosine, it is of interest to know what this SH2 domain interacts with. Is the YRC an appropriate collaborator for a 2-hybrid screen with a subdomain of a yeast protein like this?

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们有兴趣研究与酿酒酵母Spt 6结构域（酵母中唯一已知的SH 2结构域）的相互作用。Spt 6具有几个结构域，因此尚不清楚先前使用整个Spt 6蛋白的实验是否相关。由于酵母不知道有磷酸酪氨酸，它是感兴趣的，知道这个SH 2结构域相互作用。YRC是否是一个合适的合作者，用这样的酵母蛋白质的子域进行双杂交筛选？