QUESTION OR TRAINING REQUEST FOR THE YEAST RESOURCE CENTER

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们感兴趣的是研究与S酿酒酵母Spt6(酵母中唯一已知的SH2结构域)结构域的相互作用。Spt6有几个结构域，因此还不清楚之前使用完整Spt6蛋白的实验是否相关。由于酵母中不存在磷酸酪氨酸，因此了解SH2结构域与其相互作用是很有意义的。对于像这样酵母蛋白亚域的双杂交筛选，YRC是合适的合作者吗？

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested in studying interactions with a structural domain of S cerevisiae Spt6 (the only known SH2 domain in yeast). Spt6 has several structural domains, so it is not clear that previous experiments that used the whole Spt6 protein will be relevant. As yeast are not known to have phosphotyrosine, it is of interest to know what this SH2 domain interacts with. Is the YRC an appropriate collaborator for a 2-hybrid screen with a subdomain of a yeast protein like this?