IDENTIFICATION OF PAX7 INTERACTING PROTEINS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 Pax 7是骨骼肌再生中卫星细胞功能所必需的肌肉转录因子。 Pax 7蛋白的水平在转录后受到调节，并影响卫星细胞自我更新或致力于肌源性分化的决定。 这一决定的一部分似乎是由卫星细胞中MyoD和肌细胞生成素的水平决定的。 高Pax 7水平诱导MyoD降解，高MyoD水平导致Pax 7降解。 我们假设这些蛋白质竞争DNA结合和稳定所需的共同因子。 这项合作的目标是鉴定增殖骨骼肌细胞中与Pax 7直接或间接相互作用的蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pax7 is a muscle transcription factor necessary for satellite cell function in skeletal muscle regeneration. The levels of Pax7 protein are regulated post-transcriptionally and affect the decision of a satellite cell to self-renew or commit to myogenic differentiation. Part of this decision appears to be determined by the levels of MyoD and myogenin in satellite cells. High Pax7 levels induce MyoD degradation and high MyoD levels cause Pax7 degradation. We hypothesize that these proteins compete for a common factor that is required for DNA binding and stabilization. The goal of this collaboration is to identify proteins in proliferating skeletal muscle cells that interact directly or indirectly with Pax7.