IDENTIFICATION OF PAX7 INTERACTING PROTEINS

PAX7 相互作用蛋白的鉴定

• 批准号: 7957715
• 负责人: Bradley B Olwin
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957715
• 关键词: Affect; Biology; Cell physiology; Collaborations; Commit; Computer Retrieval of Information on Scientific Projects Database; DNA Binding; Funding; Fungal Genome; Goals; Grant; Institution; Muscle; Muscle Fibers; Myogenin; PAX7 gene; Paired box protein Pax-7; Proliferating; Proteins; Research; Research Personnel; Resources; Skeletal Muscle; Source; United States National Institutes of Health; muscle regeneration; satellite cell; self-renewal; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Pax7 is a muscle transcription factor necessary for satellite cell function in skeletal muscle regeneration. The levels of Pax7 protein are regulated post-transcriptionally and affect the decision of a satellite cell to self-renew or commit to myogenic differentiation. Part of this decision appears to be determined by the levels of MyoD and myogenin in satellite cells. High Pax7 levels induce MyoD degradation and high MyoD levels cause Pax7 degradation. We hypothesize that these proteins compete for a common factor that is required for DNA binding and stabilization. The goal of this collaboration is to identify proteins in proliferating skeletal muscle cells that interact directly or indirectly with Pax7.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 PAX7是骨骼肌再生过程中卫星细胞功能所必需的一种肌肉转录因子。Pax7蛋白的水平受转录后调控，影响卫星细胞自我更新或致力于肌源性分化的决定。这一决定的一部分似乎是由卫星细胞中MyoD和肌肉生成素的水平决定的。高Pax7水平导致MyoD降解，高MyoD水平导致Pax7降解。我们假设这些蛋白质竞争DNA结合和稳定所需的共同因子。这项合作的目标是确定增殖的骨骼肌细胞中直接或间接与Pax7相互作用的蛋白质。