CYST WALL ENDOPROTEASES OF ENTAMOEBA INVADENS AND ENTAMOEBA HISTOLYTICA

入侵内阿米巴和溶组织内阿米巴的囊壁内切蛋白酶

• 批准号: 7955937
• 负责人: John C. Samuelson
• 金额: $ 0.47万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955937
• 关键词: Alpha-mannosidase; Amebic colitis; Anabolism; Anti-Retroviral Agents; Biochemical; Biology; Complex; Computer Retrieval of Information on Scientific Projects Database; Concanavalin A; Cyanovirin-N; Cyst; Endoplasmic Reticulum; Entamoeba histolytica; Entamoeba invadens; Funding; Glucosyltransferase; Glucosyltransferases; Glycoproteins; Grant; Institution; Lectin; Link; Liver Abscess; Mass Spectrum Analysis; Medicine; Methods; Polysaccharides; Quality Control; Research; Research Personnel; Resources; Source; Surface; System; United States National Institutes of Health; Upper arm; interest; protein folding; sugar nucleotide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. N-Glycans of Entamoeba histolytica, the protist that causes amebic dysentery and liver abscess, are of great interest for multiple reasons. E. histolytica makes an unusual truncated N-glycan precursor (Man(5)GlcNAc(2)), has few nucleotide sugar transporters, and has a surface that is capped by the lectin concanavalin A. Here, biochemical and mass spectrometric methods were used to examine N-glycan biosynthesis and the final N-glycans of E. histolytica with the following conclusions. Unprocessed Man(5)GlcNAc(2), which is the most abundant E. histolytica N-glycan, is aggregated into caps on the surface of E. histolytica by the N-glycan-specific, anti-retroviral lectin cyanovirin-N. Glc(1)Man(5)GlcNAc(2), which is made by a UDP-Glc: glycoprotein glucosyltransferase that is part of a conserved N-glycan-dependent endoplasmic reticulum quality control system for protein folding, is also present in mature N-glycans. A swainsonine-sensitive alpha-mannosidase trims some N-glycans to biantennary Man(3)GlcNAc(2). Complex N-glycans of E. histolytica are made by the addition of alpha1,2-linked Gal to both arms of small oligomannose glycans, and Gal residues are capped by one or more Glc. In summary, E. histolytica N-glycans include unprocessed Man(5)GlcNAc(2), which is a target for cyanovirin-N, as well as unique, complex N-glycans containing Gal and Glc.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 溶组织内阿米巴是引起阿米巴痢疾和肝脓肿的原生生物，其N-糖链引起人们的极大兴趣，原因有很多。溶组乳杆菌产生一种不寻常的截短的N-糖前体(Man(5)GlcNAc(2))，几乎没有核苷酸糖转运体，其表面被刀豆蛋白A所覆盖。未加工的人(5)GlcNAc(2)是最丰富的N-糖链，通过N-糖特异性的抗逆转录病毒凝集素氰病毒素-N聚集在组织链球菌胞外膜上。GLC(1)Man(5)GlcNAc(2)是由UDP-GLC：糖蛋白葡萄糖转移酶产生的，它是保守的N-糖依赖的内质网蛋白质折叠质量控制系统的一部分，也存在于成熟的N-糖中。苦马豆素敏感的α-甘露糖苷酶将一些N-糖链修剪成双天线的Man(3)GlcNAc(2)。溶组埃希菌的复杂N-糖链是通过在小分子寡聚糖的两个臂上添加α-1，2-连接的半乳糖而形成的，半乳糖残基由一个或多个糖链封端。总之，溶解组织杆菌的N-聚糖包括未加工的Man(5)GlcNAc(2)，它是氰基韦林-N的靶标，以及含有Gal和GLC的独特的复杂N-聚糖。