ADVANCED CELL AND TISSUE ENGINEERING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该课程面向参与协作项目的个人。培训的重点是动手操作所有基本技能，使个人能够进行涉及使用培养细胞和/或工程组织的实验。 目前的培训包括：基本无菌细胞培养技术，细胞保存和恢复方法，细胞计数，玫瑰小室细胞培养，人正常真皮和病理性真皮组织的分离，人胶质母细胞瘤建立细胞系以供将来使用，肿瘤球体和胶原和纤维蛋白的RAFT培养。此外，我们还提供了无壳鸡胚胎CAM(绒毛尿囊膜)模型的培训，并植入了肿瘤球体。TUNEL法、肌动蛋白显像法和各种其他标记方法，允许单独使用MPM或MPM/OCT成像特定的亚细胞细胞器和中间丝，目的是监测治疗的结果和细胞外基质中细胞功能的动态变化。 在过去的一年里，我们发展了我们的培训课程。我们在2006年12月中旬接受了第一例人脑胶质母细胞瘤活组织检查(工作正在进行中)，开始了肿瘤干细胞的分离，特别关注人胶质母细胞瘤CD133阳性细胞。采集建立无壳鸡胚CAM模型过程的数字图像并用于训练。从其他机构培训的缔约方广泛要求提供这些图像。我们完整的木筏养殖方案也得到了广泛的要求。我们已经收到了许多请求，并为建立肿瘤球体提供了培训。在未来的一年里，我们将重点在网上发布所有现有的协议，并附上数字图像，供场外合作者通过密码访问和内部自我指导培训访问。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The class is offered to individuals involved in collaborative projects. The training focuses on the hands-on manipulation of all essential skills to allow the individual to carry out experiments that involve the use of cultures cells and/or engineered tissues. Current training includes: Basic sterile cell culture techniques, methods of cell preservation and recovery, cell counting, culture of cells in Rose Chambers, dissociation of human tissues of normal dermal and pathological dermal in origin and human glioblastoma to establish cell lines for future use, tumor spheroid and raft culture in collagen and fibrin. In addition, we provide training in Shellless Chick Embryo CAM (Chorioallantoic Membrane) model with tumor spheroids implantation. TUNEL assay, actin visualization and various other labeling methods that allows the MPM alone or MPM/OCT imaging of specific subcellular organelles and intermediate filaments with the purpose of monitoring the consequence of therapeutically treatment and dynamics of cell function in extracellular matrix. In the past year we have developed our training classes. We started tumor stem cells isolation with special focus on human glioblastoma CD133 positive cells with the first human glioblastoma biopsy received in mid-December 2006 (work in progress). Digital images of the procedure to establish Shellless Chick Embryo CAM models were acquired and used for training. These images were widely requested by parties trained from other institutions. Our complete Raft culture protocol has also been widely requested. We have received many requests and have provided training for establishment of tumor spheroids. In the coming year we will focus on posting all existing protocols on-line with digital images attached for access by off site collaborators with password access and for in house self guided training.