STRUCTURAL STUDIES OF RNASE MRP
STRUCTURAL STUDIES OF RNASE MRP
批准号:
7957302
负责人:
ANDREY S. KRASILNIKOV
金额:
$0.48万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-07-01 至 2010-06-30
关键词:
Binding SitesCatalysisComplexComputer Retrieval of Information on Scientific Projects DatabaseCrystallographyEndoribonucleasesEnzymesFundingGrantHoloenzymesIndividualInstitutionLightMetabolismProteinsRNARNA BindingRNA-Protein InteractionRNAse MRPReportingResearchResearch PersonnelResolutionResourcesRibonucleasesRibonucleoproteinsRibosomal RNARoleSiteSourceStructureSynchrotronsUnited States National Institutes of Healthbaseendoribonucleasenumb protein
中文摘要
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
核糖核酸酶MRP(RNase MRP)是一种位点特异性内切核酸酶,参与包括rRNA在内的多种RNA分子的代谢。核糖核酸酶MRP是一种核糖核蛋白复合体,它包含一个大的RNA分子和许多蛋白质组分。RNase MRP的RNA部分被认为是起催化作用的,而蛋白质组分的作用尚不清楚。阻碍对该酶进一步了解的主要问题是缺乏可用的高分辨率结构信息。目前,还没有关于RNaseMRP任何部分的结构的报道。我们的研究旨在获得关于单个组分或亚复合体的高分辨率结构信息,并最终获得RNase MRP的全酶。最近,我们已经确定RNase MRP的两个蛋白质组分Pop6和Pop7形成一个异源二聚体,与RNase MRP的RNA组分结合。我们制备了含有Pop6、Pop7及其在RNA上的结合位置的三重络合物的晶体。我们将解开这个复合体的晶体结构,以阐明这类重要的催化核糖核蛋白复合体中的RNA-蛋白质相互作用。该结构将揭示蛋白质和RNA分子在这一重要的RNA酶类中的相互作用。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Ribonuclease MRP (RNase MRP) is a site-specific endoribonuclease involved in metabolism of various RNA molecules including rRNA. RNase MRP is a ribonucleoprotein complex which contains a large RNA molecule and a number of protein components. The RNA moiety of RNase MRP is presumed be responsible for catalysis, while the role of the protein components is not clear. The major problem that hinders further understanding of this enzyme is the absence of available high-resolution structural information. At this moment, there are no reported structures of any part of RNase MRP. Our research is aimed at obtaining high-resolution structural information on the individual components or subcomplexes and, eventually, the holoenzyme of RNase MRP. Recently, we have established that two of the protein components of RNase MRP, Pop6 and Pop7, form a heterodimer which binds the RNA component of RNase MRP. We produced crystals of this triple complex containing Pop6, Pop7 and their binding site on RNA. We will solve the crystal structure of this complex to shed light on the RNA-protein interactions in this important class of catalytic ribonucleoprotein complexes. The structure will reveal the interplay between protein and RNA molecules in this important class of RNA-based enzymes.
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会议论文
Structural and functional studies of RNase MRP RNP
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批准号:10221001
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项目类别:
-
资助金额:$34.37万
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财政年份:2019
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负责人:ANDREY S. KRASILNIKOV
-
依托单位:
Structural and functional studies of RNase MRP RNP
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批准号:10017277
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项目类别:
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资助金额:$34.37万
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财政年份:2019
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Structural and functional studies of RNase MRP RNP
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批准号:10439791
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项目类别:
-
资助金额:$34.37万
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财政年份:2019
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Proteins in RNA-Based Enzymes
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批准号:7924979
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项目类别:
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资助金额:$19.96万
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财政年份:2009
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Proteins in RNA-based Enzymes
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批准号:8694771
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项目类别:
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资助金额:$31.4万
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财政年份:2008
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负责人:ANDREY S. KRASILNIKOV
-
依托单位:
Proteins in RNA-Based Enzymes
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批准号:7506024
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项目类别:
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资助金额:$28.76万
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财政年份:2008
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Proteins in RNA-Based Enzymes
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批准号:8269726
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项目类别:
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资助金额:$28.29万
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财政年份:2008
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Proteins in RNA-Based Enzymes
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批准号:8074381
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项目类别:
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资助金额:$28.29万
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财政年份:2008
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Proteins in RNA-based Enzymes
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批准号:9068156
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项目类别:
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资助金额:$31.4万
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财政年份:2008
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Proteins in RNA-Based Enzymes
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批准号:7656666
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项目类别:
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资助金额:$28.85万
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财政年份:2008
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
Proteins in RNA-Based Enzymes
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批准号:7848372
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项目类别:
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资助金额:$28.57万
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财政年份:2008
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负责人:ANDREY S. KRASILNIKOV
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依托单位:
国内基金
海外基金
不对称Tandem catalysis 合成手性仲醇
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批准号:20643008
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项目类别:专项基金项目
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资助金额:8.0万元
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批准年份:2006
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负责人:孙伟
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依托单位: