STRUCTURE OF COPII COATED VESICLES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 真核细胞的内膜是动态结构，其通过专门的细胞机制如外壳蛋白复合物II（COPII）的活性持续通信，所述外壳蛋白复合物II（COPII）介导从内质网（ER）输出货物。COPII由Sar 1 GT3、Sec 23/Sec 24（Sec 23/24）和Sec 13/Sec 31（Sec 13/31）组成，其中Sec 23是Sar 1特异性GT3活化蛋白，Sec 24在货物选择中起作用，Sec 13/Sec 31具有结构作用。虽然最近的研究结果表明，Sec 23/24和Sec 13/31可以自组装形成COPII笼状颗粒，但我们现在已经证明，Sec 13/31可以在不存在Sec 23/24的情况下自组装形成最小笼。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Endomembranes of eukaryotic cells are dynamic structures that are in continuous communication through the activity of specialized cellular machineries such as the coat protein complex II (COPII), which mediates cargo export from the endoplasmic reticulum (ER). COPII is comprised of the Sar1 GTPase, Sec23/Sec24 (Sec23/24), where Sec23 is a Sar1-specific GTPase activating protein and Sec24 functions in cargo selection, and Sec13/Sec31 (Sec13/31), which has a structural role. While recent results showed that Sec23/24 and Sec13/31 can self-assemble to form COPII cage-like particles, we have now shown that Sec13/31 can self-assemble to form minimal cages in the absence of Sec23/24.