EFFECTS OF SPECIFIC SPERMATID-EXPRESSED Y CHROMOSOME GENES ON SPERM FUNCTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Y染色体曾经被认为只负责通过睾丸决定基因Sry向雄性途径开启性别决定。 然而，随后的研究揭示了位于该染色体上的许多其他基因，这些基因可能编码对雄性配子的形成和功能重要的信息。 Y染色体曾经被认为只负责通过睾丸决定基因Sry向雄性途径开启性别决定。 然而，随后的研究揭示了位于该染色体上的许多其他基因，这些基因可能编码对雄性配子的形成和功能重要的信息。 该项目的目标是确定某些Y编码基因的作用。 更具体地说，我们试图（1）揭示Y染色体长臂上编码的基因的功能和作用机制，（2）测试睾丸决定基因Sry是否在精子功能中起作用;（3）定义足以产生能够实现成功受精的配子的最小Y基因互补。 这将通过分析缺乏Y染色体各部分的独特小鼠模型，并使用辅助生殖技术（ART）：卵胞浆内单精子注射（ICSI）和体外受精（IVF），结合分子生物学方法来完成。 男性特定区域的Y染色体缺失是生精失败的常见遗传原因，使携带受影响Y染色体的男性成为通过ART治疗不孕症的目标群体。鉴于与人类ART使用相关的已知和潜在问题，必须继续努力确定不孕症的根本原因。 我们的工作将进一步了解Y染色体编码基因在精子发生和精子功能中的作用，最终可能有助于对抗不孕症，这是美国的一个主要健康问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The Y chromosome was once thought to be responsible only for turning on sex determination towards the male pathway via the testis determinant gene Sry. However, subsequent studies revealed a number of other genes located on this chromosome that are likely to encode information important for formation and function of the male gametes. The goal of this project is to define the roles of certain Y encoded genes. More specifically, we seek to (1) unveil the function and mechanism of action of genes encoded on the Y chromosome long arm, (2) test whether testis determinant gene Sry plays a role in sperm function; (3) define the minimum Y gene complement that is sufficient to generate gametes capable of achieving successful fertilization. This will be done through the analysis of unique mouse models lacking various parts of the Y chromosome, and use of assisted reproduction technologies (ART): intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF), in combination with molecular biology approaches. Deletions within the male specific region of the Y chromosome are a common genetic cause of spermatogenic failure, making men carrying affected Y chromosome a target group for infertility treatment via ART. Given the known and potential problems associated with the use of ART in humans, it is essential to continue efforts on identifying the underlying causes of infertility. Our work will advance understanding of the Y chromosome encoded genes role in spermatogenesis and sperm function and ultimately may help to combat infertility, a major health problem in U.S.A.