COBRE: UNV MED SCH: CORE C: CELL PROTEOMICS INTERFACE FACILITY

COBRE：UNV MED SCH：Core C：细胞蛋白质组学接口设施

• 批准号: 7960571
• 负责人: Christine R Cremo
• 金额: $ 19.32万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960571
• 关键词: Bioinformatics; Centers of Research Excellence; Collaborations; Communities; Computer Retrieval of Information on Scientific Projects Database; Computing Methodologies; Core Facility; Cultured Cells; Data Analyses; Databases; Funding; Grant; Human Resources; Institution; Lead; Nevada; Operative Surgical Procedures; Post-Translational Protein Processing; Preparation; Proteins; Proteomics; Relative (related person); Research; Research Personnel; Resources; Smooth Muscle; Source; Structure of thyroid parafollicular cell; Subcellular Fractions; Techniques; Time; Tissues; United States National Institutes of Health; Work; Workload; meetings

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The COBRE needs personnel to interface with two Nevada core facilities here at UNR, Proteomics and Bioinformatics, to enhance our ability to complete aims related to proteomics approaches. The two Nevada cores do not have sufficient staff to fully meet the needs for the COBRE. The core will serve to relieve the workload on the P. I.s by allowing them to focus upon the main aims of their projects and at the same time explore new directions which will lead to new questions. Overview of Core Operations. The core will serve as technical support for the 5 projects. The main functions of the core are as follows. 1) Work with the Core Director and each P. I. to develop a detailed experimental approach for proteomics component of each project. 2) Facilitate the preparation of reproducible subcellular fractions from tissues or cultured cells that are appropriate to the question. 3) Use appropriate techniques to identify, determine relative amounts, and post-translational modifications of proteins in these subcellular fractions, in collaboration with the Nevada Proteomics Core Facility. 4) Assistance with mass spectral data analysis in collaboration with the Nevada Proteomics Core Facility. 5) Assistance with computational methods to annotate and identify function of newly discovered proteins, in collaboration with the Nevada Bioinformatics Core. 6) Assistance with how to incorporate the information obtained into the database and provide access to the biomedical community, in collaboration with the Nevada Bioinformatics Core.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 COBRE需要人员与UNR的两个内华达州核心设施，蛋白质组学和生物信息学进行对接，以提高我们完成与蛋白质组学方法相关目标的能力。两个内华达州核心没有足够的工作人员来完全满足COBRE的需求。核心将有助于减轻P.I. S的工作量，使他们能够专注于他们项目的主要目标，同时探索新的方向，这将导致新的问题。核心业务概览。该核心将为5个项目提供技术支持。核心的主要功能如下。1)与核心总监和每个私人侦探合作。为每个项目的蛋白质组学部分制定详细的实验方法。2)促进从组织或培养细胞中制备适用于该问题的可重现亚细胞组分。3)与内华达州蛋白质组学核心设施合作，使用适当的技术来鉴定、测定这些亚细胞组分中蛋白质的相对量和翻译后修饰。4)协助与内华达州蛋白质组学核心设施合作进行质谱数据分析。5)与内华达州生物信息学中心合作，协助计算方法注释和鉴定新发现的蛋白质的功能。6)与内华达州生物信息学核心合作，协助如何将获得的信息纳入数据库，并提供对生物医学界的访问。