Gene Expression Shared Resource

基因表达共享资源

• 批准号: 7944593
• 负责人: STANLEY F. NELSON
• 金额: $ 26.67万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-02 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944593
• 关键词: Advisory Committees; Animal Model; Archives; Area; Base Sequence; Behavior; Biological Assay; Biometry; Budgets; Cancer Center; Cancer Center Support Grant; Cancer cell line; Characteristics; Clinical; Commit; Communities; Complement; Complex; DNA; DNA copy number; DNA-Binding Proteins; Data; Data Analyses; Deposition; Detection; Development; Dissection; Epidemiology; Equipment; Faculty; Gene Expression; Genes; Genetic; Genome; Genomics; Genotype; Grant; Housing; Individual; Informatics; Jonsson Comprehensive Cancer Center of University of California Los Angeles; Laboratories; Leadership; Link; Malignant Neoplasms; Medical Research; Methylation; Microarray Analysis; Monitor; Online Systems; Organism; Performance; Play; Recording of previous events; Regulation; Reporting; Research; Research Personnel; Resource Sharing; Resources; Role; Sampling; Services; Source; Specimen; Structure; System; Technology; Time; Training; Transcriptional Regulation; Triage; Variant; Vendor; base; computer infrastructure; data management; density; design; experience; genetic analysis; laboratory facility; member; new technology; next generation; novel; programs; promoter; research study

The Gene Expression Shared Resource (GESRV was first proposed as a JCCC Shared Resource for the 2002 competing renewal application. The GESR was established to provide broad access to genome-scale gene expression analysis to JCCC investigators who are using a variety of organisms and who wish to implement microarray technology into their research programs. It is now a well-established, broadly-used Shared Resource within the JCCC. Currently, the GESH houses equipment and provides technical and intellectual expertise to guide faculty in the performance of genomic analyses. The Shared Resource has provided access to microarray based genomic analysis for gene expression, copy number, ChlP-Chip and genotyping. The GESR houses equipment for Affymetrix and Agilent based experiments. In addition, the Shared Resource maintains all data online for ease of analysis and provides links to analytical resources, both within the GESR and within the JCCC BASE Unit Shared Resource. The GESR wet laboratory facility is adjacent to the laboratory of the Director. A mature system is in place for recharge-based support of Shared Resource activities. The GESR assures JCCC investigators of timely access to genomic technology and provides access to intellectual expertise provided by the co-directors and staff. The GESR adapts to new technologies, including next generation sequencing; hardware is in place and the GESR has established the required computational infrastructure for sequence-based gene expression analysis. During the last reporting year of the current grant cycle, the GESR performed nearly 2000 analyses for 38 JCCC members; 82 percent of the studies performed in the GESR during this period were for JCCC members. Twenty percent of the proposed operating budget for the Gene Expression Shared Resource is requested from the JCCC CCSG application. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). 38 Cancer Center members representing seven Cancer Center Program Areas utilized the services of the Gene Expression Shared Resource during the reporting period. This is a continuing shared resource.

基因表达共享资源（GESRV）首先被提出作为JCCC共享资源， 2002年竞争性续期申请。GESR的建立是为了提供广泛的基因组规模的 基因表达分析，以JCCC研究人员谁是使用各种生物，谁希望 将微阵列技术应用到他们的研究项目中。它现在是一个成熟的，广泛使用的 JCCC内的共享资源。目前，GESH拥有设备，并提供技术和 知识专长，以指导教师在基因组分析的性能。共享资源具有 提供了对基因表达、拷贝数、ChIP-芯片和基于微阵列的基因组分析的访问。 基因分型GESR包含用于Affyoung和Agilent实验的设备。此外该 共享资源在线维护所有数据以便于分析，并提供分析资源的链接， 在GESR和JCCC基础单元共享资源内。GESR湿实验室设施是 与处长的化验所毗邻。为基于充电的共享支持提供了成熟的系统 资源活动。GESR确保JCCC研究人员及时获得基因组技术， 提供获得共同主任和工作人员提供的知识专长的机会。GESR适应新的 技术，包括下一代测序;硬件已经到位，GESR已经建立了 基于序列的基因表达分析所需的计算基础设施。在过去 在当前资助周期的报告年度，GESR对38个JCCC成员进行了近2000次分析; 在此期间，GESR进行的研究中有82%是针对JCCC成员的。二十 基因表达共享资源的拟议业务预算的%是从 JCCC CCSG应用程序。（另请参见第6.2.3节关于共享资源的历史，描述， 基本特征）。 代表七个癌症中心项目领域的38名癌症中心成员使用了 基因表达共享资源在报告期内。这是一个持续的共享 resource.