Roche NimbleGen MS200 2 Micron Microarray Scanner

Roche NimbleGen MS200 2 微米微阵列扫描仪

• 批准号: 7793240
• 负责人: Sandra Austin-Phillips
• 金额: $ 12.82万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-20 至 2011-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793240
• 关键词: Adoption; Agreement; Arts; Axon; Binding; Biotechnology; Chromatin Structure; Copy Number Polymorphism; DNA; DNA Methylation; DNA Microarray Chip; Data; Ensure; Fee-for-Service Plans; Funding; Gene Expression; Generations; Genomics; Goals; Knowledge; Laboratories; Medical Research; Methods; Microarray Analysis; Price; Research; Research Personnel; Resolution; Services; Technology; United States National Institutes of Health; Universities; Wisconsin; Work; cost; cost effective; density; improved; instrument; instrumentation; microbial; novel; small molecule; transcription factor

DESCRIPTION (provided by applicant): We are requesting funds to acquire a Roche NimbleGen MS200 25m Microarray Scanner. The instrument will be placed in the University of Wisconsin Biotechnology Center's Gene Expression Center (GEC). The GEC was established over ten years ago as a central fee-for-service core service facility at UW-Madison for global gene expression analysis using state-of-the-art DNA microarray technologies. This scanner is needed to capture data from high-density arrays such as Roche NimbleGen High Density arrays (HD2). Roche NimbleGen's MAS (Maskless Array Synthesis) technology offers a unique ability to make high-density arrays for advanced genomic analysis methods such as CGH, ChIP-chip, microbial resequencing, DNA methylation and gene expression. With 2.1 million probes, NimbleGen HD2 arrays offer the opportunity to fully interrogate copy number variation, transcription factor binding, and chromatin structure. The UW-Madison also has a preferred pricing agreement for Roche NimbleGen array purchases that makes the use of this particular HD array technology very cost effective to federally funded researchers. The GEC also currently offers subsidized comprehensive services that allow investigators not only to fully utilize Nimblegen MAS arrays but also to explore and improve upon their standard capabilities. In addition some investigators are developing exciting novel high-density platforms such as DNA and small molecule arrays and local access to a HD scanner is essential for this work. The currently available Axon 4000B scanner at the GEC does not have the required resolution needed for data capture from the new generation of recently introduced HD2 arrays and to our knowledge there is not a scanner that offers this resolution available to researchers on campus. In this proposal we will highlight seven laboratories out of many funded by NIH at the UW-Madison whose research would benefit from using high-density arrays and/or access to the high-resolution scanner. Our goal however, is to provide access to this relatively new technological platform to all NIH funded researchers at UW-Madison. This facilitated access will accelerate both the pace and scope of basic medical research at the UW. Placing this instrumentation in a central core service facility will provide equitable cost-effective access to this technological platform and also ensure that the instrument is heavily used and well maintained. Local access also facilitates adoption of this new genomics platform by investigators currently unfamiliar with the capabilities of this instrument.

描述（由申请人提供）： 我们正在申请资金购买 Roche NimbleGen MS200 25m 微阵列扫描仪。该仪器将放置在威斯康星大学生物技术中心的基因表达中心（GEC）。 GEC 成立于十多年前，是威斯康辛大学麦迪逊分校的一个按服务收费的核心服务机构，利用最先进的 DNA 微阵列技术进行全球基因表达分析。该扫描仪需要从高密度阵列（例如 Roche NimbleGen 高密度阵列 (HD2)）捕获数据。罗氏 NimbleGen 的 MAS（无掩模阵列合成）技术具有独特的能力，可以为先进的基因组分析方法（例如 CGH、ChIP 芯片、微生物重测序、DNA 甲基化和基因表达）制造高密度阵列。 NimbleGen HD2 阵列拥有 210 万个探针，为全面探究拷贝数变异、转录因子结合和染色质结构提供了机会。威斯康星大学麦迪逊分校还与罗氏 NimbleGen 阵列采购签订了优先定价协议，这使得使用这种特殊的 HD 阵列技术对于联邦资助的研究人员来说非常具有成本效益。 GEC 目前还提供有补贴的综合服务，使研究人员不仅可以充分利用 Nimblegen MAS 阵列，还可以探索和改进其标准功能。此外，一些研究人员正在开发令人兴奋的新型高密度平台，例如 DNA 和小分子阵列，并且本地访问高清扫描仪对于这项工作至关重要。 GEC 当前可用的 Axon 4000B 扫描仪不具备从最近推出的新一代 HD2 阵列采集数据所需的分辨率，并且据我们所知，没有扫描仪可为校园研究人员提供此分辨率。在本提案中，我们将重点介绍 NIH 资助的威斯康辛大学麦迪逊分校的七个实验室，这些实验室的研究将受益于使用高密度阵列和/或使用高分辨率扫描仪。然而，我们的目标是为威斯康辛大学麦迪逊分校所有 NIH 资助的研究人员提供这个相对较新的技术平台。这种便利的访问将加快华盛顿大学基础医学研究的步伐和范围。将该仪器放置在中央核心服务设施中将为该技术平台提供公平且具有成本效益的访问，并确保该仪器得到大量使用和良好维护。本地访问还有助于目前不熟悉该仪器功能的研究人员采用这种新的基因组学平台。