Roche NimbleGen MS200 2 Micron Microarray Scanner

描述（由申请人提供）： 我们正在申请资金购买罗氏NimbleGen MS 200 25米微阵列扫描仪。该仪器将被放置在威斯康星州大学生物技术中心的基因表达中心（GEC）。GEC成立于十多年前，是威斯康星大学麦迪逊分校的一个中央收费服务核心服务设施，使用最先进的DNA微阵列技术进行全球基因表达分析。该扫描仪需要从高密度阵列（如Roche NimbleGen高密度阵列（HD2））中捕获数据。Roche NimbleGen的MAS（无掩模阵列合成）技术提供了一种独特的能力，可以为先进的基因组分析方法（如CGH、ChIP芯片、微生物重测序、DNA甲基化和基因表达）制作高密度阵列。NimbleGen HD2阵列拥有210万个探针，可全面查询拷贝数变异、转录因子结合和染色质结构。UW-Madison还为Roche NimbleGen阵列购买提供了优先定价协议，这使得使用这种特定的HD阵列技术对联邦资助的研究人员来说非常具有成本效益。GEC目前还提供有补贴的综合服务，使调查人员不仅能够充分利用Nimblegen MAS阵列，而且还能够探索和改进其标准能力。此外，一些研究人员正在开发令人兴奋的新型高密度平台，如DNA和小分子阵列，本地访问高清扫描仪对这项工作至关重要。GEC目前可用的Axon 4000 B扫描仪不具有从最近推出的新一代HD2阵列捕获数据所需的分辨率，据我们所知，校园内的研究人员还没有提供这种分辨率的扫描仪。在本提案中，我们将重点介绍美国国立卫生研究院在威斯康星大学麦迪逊分校资助的许多实验室中的七个实验室，这些实验室的研究将受益于使用高密度阵列和/或使用高分辨率扫描仪。然而，我们的目标是提供访问这个相对较新的技术平台，所有NIH资助的研究人员在威斯康星大学麦迪逊分校。这种便利的访问将加快华盛顿大学基础医学研究的步伐和范围。将这一仪器放置在中央核心服务设施中，将为使用这一技术平台提供公平的成本效益，并确保仪器得到大量使用和良好维护。本地访问还有助于目前不熟悉该仪器功能的研究人员采用这一新的基因组学平台。

DESCRIPTION (provided by applicant): We are requesting funds to acquire a Roche NimbleGen MS200 25m Microarray Scanner. The instrument will be placed in the University of Wisconsin Biotechnology Center's Gene Expression Center (GEC). The GEC was established over ten years ago as a central fee-for-service core service facility at UW-Madison for global gene expression analysis using state-of-the-art DNA microarray technologies. This scanner is needed to capture data from high-density arrays such as Roche NimbleGen High Density arrays (HD2). Roche NimbleGen's MAS (Maskless Array Synthesis) technology offers a unique ability to make high-density arrays for advanced genomic analysis methods such as CGH, ChIP-chip, microbial resequencing, DNA methylation and gene expression. With 2.1 million probes, NimbleGen HD2 arrays offer the opportunity to fully interrogate copy number variation, transcription factor binding, and chromatin structure. The UW-Madison also has a preferred pricing agreement for Roche NimbleGen array purchases that makes the use of this particular HD array technology very cost effective to federally funded researchers. The GEC also currently offers subsidized comprehensive services that allow investigators not only to fully utilize Nimblegen MAS arrays but also to explore and improve upon their standard capabilities. In addition some investigators are developing exciting novel high-density platforms such as DNA and small molecule arrays and local access to a HD scanner is essential for this work. The currently available Axon 4000B scanner at the GEC does not have the required resolution needed for data capture from the new generation of recently introduced HD2 arrays and to our knowledge there is not a scanner that offers this resolution available to researchers on campus. In this proposal we will highlight seven laboratories out of many funded by NIH at the UW-Madison whose research would benefit from using high-density arrays and/or access to the high-resolution scanner. Our goal however, is to provide access to this relatively new technological platform to all NIH funded researchers at UW-Madison. This facilitated access will accelerate both the pace and scope of basic medical research at the UW. Placing this instrumentation in a central core service facility will provide equitable cost-effective access to this technological platform and also ensure that the instrument is heavily used and well maintained. Local access also facilitates adoption of this new genomics platform by investigators currently unfamiliar with the capabilities of this instrument.