Spinning Disk Confocal for Live Cell Imaging with Accessories

描述（由申请人提供）：我们正在申请资金，用于在哈佛神经发现中心（HNDC）光学成像核心（OIC）建立先进的活细胞成像系统。HNDC是一个由来自更广泛的哈佛社区的研究人员组成的社区，包括哈佛医学院（HMS）及其在大波士顿地区的18家附属教学医院。HNDC的成员对基础和应用神经科学研究感兴趣，这些研究为阿尔茨海默病、帕金森病、肌萎缩侧索硬化症和多发性硬化症等神经退行性疾病的病因、预防和治疗提供了见解。HNDC光学成像核心与来自我们社区的100多个研究小组合作并为其提供服务；其中大部分是由小灵通项目资助的。尽管提供了一系列先进的光学成像和图像分析服务，但该中心的光学成像核心具有非常有限的活细胞成像能力（由基于狭缝的奥林巴斯DSU单元，弧光灯光源和小阶段孵化器组成）。我们的许多用户寻求访问高性能活细胞成像系统。经过与用户的广泛研究和讨论，我们设计了一种独特的活细胞成像装置，该装置建立在我们现有的奥林巴斯磁盘旋转单元(DSU) - IX81显微镜系统之上。升级包括一个高性能的Andor Revolution XD共聚焦系统与横河CSU- X1扫描仪单元，多个激光激发线，和必要的活细胞成像配件。通过建立在我们现有的平台上（并避免需要购买新的显微镜体），这个建议的设计以一种经济有效的方式解决了我们用户提出的主要问题。此外，通过将奥林巴斯DSU与横河CSU-X1扫描仪单元相结合，我们将提供超越任何市售的现成共聚焦系统的活细胞成像能力。建立这样一个多功能，高性能的活细胞成像系统将对我们非常广泛的phs资助用户群体的研究产生巨大影响。

DESCRIPTION (provided by applicant): We are requesting funds to establish an advanced live cell imaging system at the Harvard NeuroDiscovery Center (HNDC) Optical Imaging Core (OIC). The HNDC is a community of researchers from across the broader Harvard community, including Harvard Medical School (HMS) and its eighteen affiliated teaching hospitals throughout the greater Boston area. Members of the HNDC are interested in both basic and applied neuroscience research that provides insight into the causes, prevention and treatment of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease, ALS and Multiple Sclerosis. The HNDC Optical Imaging Core collaborates with and serves over 100 research groups from across our community; the majority of which are funded by PHS programs. Despite providing a range of advanced optical imaging and image analysis services, the Center's Optical Imaging Core has a very limited live cell imaging capability (consisting of slit- based Olympus DSU unit, Arc-lamp light sources and a small stage incubator). Many of our users seek access to a high performance live cell imaging system. After extensive research and discussion with our users, we have designed a unique live cell imaging setup that builds on our existing Olympus Disk Spinning Unit (DSU) - IX81 microscope system. The upgrade includes a high performance Andor Revolution XD confocal system with the Yokogawa CSU- X1 scanner unit, multiple laser excitation lines, and essential live cell imaging accessories. By building upon our existing platform (and avoiding the need to purchase a new microscope body), this proposed design addresses the major issues raised by our users in a cost-effective way. Furthermore, by combining the Olympus DSU with the Yokogawa CSU-X1 scanner unit, we will provide a live cell imaging capability beyond that of any commercially available off-the- shelf confocal system. Establishing such a versatile, high-performance live cell imaging system will have an enormous impact on the research of our very broad PHS-funded user group.