ZEISS Spinning Disc/LSM700 Point Scanning Confocal Microscope with Incubation Sys

蔡司转盘/LSM700 点扫描共焦显微镜，带孵化系统

• 批准号: 7794517
• 负责人: CRISTINA Maria FERNANDEZ-VALLE
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CENTRAL FLORIDA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794517
• 关键词: Area; Award; Biomedical Research; Cardiovascular Diseases; Cells; Communicable Diseases; Computer software; Contract Services; Core Facility; Dyes; Faculty; Fingerprint; Florida; Fluorescence; Funding; Goals; Image; Lasers; Life; Malignant Neoplasms; Medicine; Microscope; Minor; Neurobiology; Nuclear; Recruitment Activity; Request for Proposals; Research; Research Personnel; Scanning; Schools; Science; System; Three-Dimensional Imaging; Time; United States National Institutes of Health; Universities; bioimaging; cellular imaging; college; data acquisition; instrument; photoactivation; programs; research study

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a Zeiss Spinning Disc/LSM 700 Point Scanning Confocal Microscope Combination System for the Bioimaging Core Facility of the Burnett School Biomedical Sciences in the newly-formed College of Medicine at University of Central Florida (UCF). The system includes an integrated Zeiss LSM 700 laser scanning confocal microscope, with a cell-observer platform and a CSU-X1 spinning disc system. This instrument allows users to perform live cell time-lapse imaging, 3D imaging, multiple fluorescence and colocalization analyses, nuclear localization, emission fingerprinting, DIC, FRAP, FLIP, photoactivation and photoconversion experiments. The unique capabilities of LSM 700 include variable secondary dichroic beam splitter, FixGate main beam splitter, laser life extender, and integrated software that controls data acquisition and analysis of multiple parameters. There is a critical need for this instrument because there is only one common use confocal microscope available at UCF and in the Central Florida area. It is a Zeiss LSM 510 scanning confocal microscope that was purchased in 2000 and is the sole instrument in the existing Bioimaging Core. This microscope stand has become obsolete and will not be supported by a Zeiss service contract as of September 2009. Furthermore, the current system does not have the capability of live cell imaging or UV excitation of nuclear dyes such as DAPI. The 8 major users of this proposal have 12 NIH funded R01 projects (2 additional R01 will begin in 2009) focused on neurobiology, cardiovascular diseases, infectious diseases, and cancer. Each award has experiments that require confocal and live imaging. In addition, there are 4 minor users who have NIH-funded projects. The purchase of the microscope system will help sustain existing NIH-funded research programs and will help recruit additional outstanding biomedical researchers. This will help the unit fulfill its strategic goal defined in 2003 to recruit 34 new faculty to build a well-funded nationally recognized biomedical research program at UCF.

描述（由申请人提供）：该提案要求资金购买Zeiss旋转盘/LSM 700点扫描共聚焦显微镜组合系统，用于在中央佛罗里达大学（UCF）新成立的医学院Burnett School Biomedical Sciences的生物成像核心设施。该系统包括一个集成的Zeiss LSM 700激光扫描共聚焦显微镜，具有单元格平台和CSU-X1旋转盘系统。该仪器允许用户执行活细胞延时成像，3D成像，多个荧光和共定位分析，核定位，发射指纹，DIC，FRAP，FLAP，FLIP，光激活和光转化实验。 LSM 700的独特功能包括可变的二级二角束分配器，Fixgate主束分离器，激光寿命扩展器以及控制数据采集和分析多个参数的集成软件。该乐器非常需要，因为UCF和中部佛罗里达州只有一种常见的共聚焦显微镜。它是2000年购买的Zeiss LSM 510扫描共聚焦显微镜，是现有生物成像核心中的唯一仪器。该显微镜架已经过时了，截至2009年9月，蔡司服务合同将不支持。此外，当前系统没有实时细胞成像或核染料（例如DAPI）的紫外线激发的能力。该提案的8个主要用户有12个NIH资助的R01项目（2009年将启动2个R01），专注于神经生物学，心血管疾病，传染病和癌症。每个奖项的实验需要共凝聚和实时成像。此外，还有4个次要用户拥有NIH资助的项目。购买显微镜系统将有助于维持现有的NIH资助研究计划，并有助于招募其他出色的生物医学研究人员。这将有助于该部门实现其在2003年定义的战略目标，以招募34名新教师，以在UCF建立一项资金充足的全国认可的生物医学研究计划。