MicroBrightField Bioscience Imaging System

描述(由申请人提供)：本申请申请购买一个微亮视野生物科学成像系统，该系统由两个组件组成：NeuroLucida(http://www.mbfbioscience.com/neurolucida)和立体调查员(http://www.mbfbioscience.com/stereo-investigator/stereo-investigator).该系统将为位于伊利诺伊州斯普林菲尔德的南伊利诺伊大学医学院(SIU-SOM)的NIH资助的研究人员提供以下能力：1)旨在量化具有特定属性的细胞亚群的形态特征的细胞重建、3D映射和形态测量(NeuroLucida)；以及2)与细胞发育、凋亡、存活、再生、功能和特定蛋白质表达相关的体视学研究(Stereo Investigator)。这些成像能力对于分析和表征细胞的形态和细胞化学特性以及细胞亚群的相互连接以及形态和细胞化学特性与细胞功能和特定蛋白质表达的相关性是必不可少的。目前，SIU-SOM没有类似能力的成像系统，邻近大学的SIU-SOM教职员工也没有类似的成像系统。几个正在进行的研究项目，无论是由国家卫生研究院资助的，还是预计在2009年由国家卫生研究院资助的，都将通过在SIU-SOM提供MBF系统而得到极大的改善。在没有MBF系统的情况下，一些SIU-SOM研究人员(例如，Uteshev博士)使用他们的协作链接访问其他大学(田纳西大学卫生科学中心，田纳西大学，田纳西州孟菲斯；381英里外和肯塔基大学，列克星敦，肯塔基州；396英里外)可用的MBF系统。其他无法使用MBF系统的SIU调查人员(例如Caspary博士和Rybak博士)必须截断他们的研究建议，以排除需要使用MBF系统的实验。该提案为NIH基金购买一个MBF系统(NeuroLucida和Stereo Investigator)提供了理由。拟议中的收购将极大地有利于SIU-SOM的基础医学和临床研究项目，将拓宽SIU调查人员解决的科学问题的范围，并将提高SIU研究的效率和生产率。参与调查的人员是药理学、内科和外科的教职员工，并得到了NIH各种资金来源(NIDDK、NIDCD、NIA、NINDS)的支持。

DESCRIPTION (provided by applicant): This application requests funds to purchase one Micro Bright Field (MBF) bioscience imaging system composed of two components: Neurolucida (http://www.mbfbioscience.com/neurolucida) and Stereo Investigator (http://www.mbfbioscience.com/stereo-investigator/stereo-investigator). The system will provide NIH-funded investigators at Southern Illinois University School of Medicine (SIU-SOM) Springfield, IL with the ability to conduct: 1) cellular reconstruction, 3D mapping and morphometry aimed at quantifying morphological characteristics of subsets of cells with specific properties (Neurolucida); and 2) stereological studies related to cellular development, apoptosis, survival, regeneration, function and expression of specific proteins (Stereo Investigator). These imaging capabilities are essential for analysis and characterization of morphological and cytochemical properties of cells as well as interconnectivity of subsets of cells and correlation of morphological and cytochemical properties with cellular functions and expression of specific proteins. At present, there is no imaging system with similar capabilities available at SIU-SOM and no similar imaging systems are available to SIU-SOM faculty members at neighboring universities. Several ongoing research projects either funded by the NIH or anticipated to be funded by the NIH in 2009 would be considerably improved by the availability of a MBF system at SIU-SOM. In the absence of MBF system, some SIU-SOM investigators (e.g., Dr. Uteshev) use their collaborative links to access MBF systems available at other universities (University of Tennessee Health Science Center, Memphis, TN; 381 miles away and University of Kentucky, Lexington, KY; 396 miles away). Other SIU investigators who do not have an access to MBF systems (e.g., Drs. Caspary and Rybak) have to truncate their research proposals to exclude experiments requiring usage of a MBF system. This proposal provides justification for the request of NIH funds to purchase one MBF system (Neurolucida and Stereo Investigator). The proposed purchase will greatly benefit basic medical and clinical research programs at SIU-SOM, will broaden the spectrum of scientific questions addressed by SIU investigators, and will increase the efficiency and productivity of SIU research. The participating investigators are faculty members of the Departments of Pharmacology, Internal Medicine and Surgery and are supported by the variety of sources of NIH funding (NIDDK, NIDCD, NIA, NINDS).