Thermo TSQ Quantum Access Mass Spectrometer

描述（由申请人提供）：这是一份新的申请，要求资金支持购买Thermo TSQ量子接入质谱仪，以增强MUSC脂质分析核心实验室的功能。这个核心实验室已经运行了7年，不断开发新的方法，逐步推进了LC/MS在脂质测定和标准化定量方面的应用。它对两个NIH项目的成功资助至关重要（MUSC的鞘脂类癌症和合作机构的肝细胞凋亡），NIH/NCRR生物医学研究卓越中心（初始和竞争更新资助），以及多个个人研究者研究项目作为nci指定的MUSC Hollings癌症中心的杰出核心设施。2008年，Analytic Core Lab记录了31个MUSC用户、41个MUSC外部用户和11个国际用户的bbb14500次脂质分析。核心实验室目前依赖于三个可操作的质谱装置，所有这些装置都已满负荷使用。因此，一个由18个主要用户组成的核心小组，代表MUSC的8个部门，正在申请这笔拨款，以获得Thermo TSQ量子接入质谱仪。这一请求得到了来自全国生物医学研究机构的6个具有代表性的外部用户样本的信件的支持。分析核心实验室的技术专家包括一名具有丰富定性和定量脂质化学经验的全职教员和3名工作人员。全面的行政计划可确保适当的管理、监督、公平获取和遵守。强有力的机构承诺确保所要求的仪器和整体设施的长期运行和维护。

DESCRIPTION (provided by applicant): This is a new application requesting funds to support the acquisition of a Thermo TSQ Quantum Access mass spectrometer to enhance the function of the Lipid Analytic Core Lab at MUSC. This core lab has been in operation for 7 years, and has continually developed novel methodology and progressively advanced the application of LC/MS for the determination of lipids and standardized quantitation. It has been critical to the successful funding of two NIH program project grants (on sphingolipids in cancer at MUSC and apoptosis in liver cells at a collaborating institution), an NIH/NCRR Center of Biomedical Research Excellence (initial and competing renewal grants), and multiple individual investigator research projects as an outstanding core facility of the NCI-designated Hollings Cancer Center at MUSC. In 2008, the Analytic Core Lab logged >14,500 lipid assays for 31 MUSC users, 41 users outside MUSC, and 11 international users. The core lab currently relies on three operational mass spectrometry set ups - all are being used to capacity. Therefore, a core group of 18 major users, representing 8 departments at MUSC, are requesting this grant to acquire a Thermo TSQ Quantum Access mass spectrometer. This request is supported by letters from a representative sample of 6 outside users at biomedical research institutions across the nation. Technical expertise within the Analytic Core Lab includes a fulltime faculty member with extensive qualitative and quantitative lipid chemistry experience and 3 staff members. A comprehensive Administrative Plan ensures appropriate management, oversight, equitable access, and compliance. Strong institutional commitment ensures long term operation and maintenance of the requested instrument and overall facility. PUBLIC HEALTH RELEVANCE: The requested instrument will be used to analyze and quantify specific lipids that are important biological mediators regulating a broad range of cellular processes. Lipids function as both energy stores and signaling molecules. In addition, they form an impermeable bilayer separating the intra- and extracellular components of cells. Major lipid classes include triacylglycerols, phospholipids, glycolipids, sphingolipids, sterols, waxes and fatty acids. Because alteration of lipid function is strongly implicated in the etiology and pathogenesis of many diseases that have a very high burden of personal and social cost, e.g., atherosclerosis, Alzheimer's disease, cancer, type 2 diabetes, inflammatory disorders, infectious diseases, neurodegeneration, obesity and stroke, research in this area is vital for identifying potential therapeutic targets.