Thermo TSQ Quantum Access Mass Spectrometer

描述（由申请人提供）：这是一项新的申请，要求提供资金，以支持收购Thermo TSQ Quantum Access质谱仪，以增强MUSC脂质分析核心实验室的功能。该核心实验室已经运行了7年，并不断开发新的方法，并逐步推进LC/MS在脂质测定和标准化定量中的应用。它对成功资助两个NIH计划赠款（关于MUSC癌症中的鞘脂和合作机构肝细胞的凋亡），NIH/NCRR生物医学研究卓越中心（初始和竞争性更新赠款）以及多个个人研究者研究项目至关重要。2008年，分析核心实验室为31名MUSC用户、41名MUSC以外的用户和11名国际用户记录了超过14，500次脂质检测。核心实验室目前依赖于三个操作质谱仪设置-所有正在使用的能力。因此，一个由18个主要用户组成的核心小组，代表了MUSC的8个部门，正在申请这笔赠款，以获得Thermo TSQ Quantum Access质谱仪。这一请求得到了来自全国生物医学研究机构的6名外部用户的代表性抽样信件的支持。分析核心实验室内的技术专长包括一名具有丰富的定性和定量脂质化学经验的全职教师和3名工作人员。一个全面的管理计划确保适当的管理，监督，公平的访问和遵守。强有力的机构承诺确保所要求的仪器和整个设施的长期运行和维护。 公共卫生相关性：所要求的仪器将用于分析和定量特定的脂质，这些脂质是调节广泛细胞过程的重要生物介质。脂质作为能量储存和信号分子。此外，它们形成了一个不可渗透的双层，将细胞的细胞内和细胞外成分分开。主要的脂质种类包括三酰基甘油、磷脂、糖脂、鞘脂、甾醇、蜡和脂肪酸。由于脂质功能的改变强烈地牵涉到许多疾病的病因和发病机制，这些疾病具有非常高的个人和社会成本负担，例如，动脉粥样硬化、阿尔茨海默病、癌症、2型糖尿病、炎症性疾病、传染病、神经变性、肥胖和中风，这一领域的研究对于确定潜在的治疗靶点至关重要。

DESCRIPTION (provided by applicant): This is a new application requesting funds to support the acquisition of a Thermo TSQ Quantum Access mass spectrometer to enhance the function of the Lipid Analytic Core Lab at MUSC. This core lab has been in operation for 7 years, and has continually developed novel methodology and progressively advanced the application of LC/MS for the determination of lipids and standardized quantitation. It has been critical to the successful funding of two NIH program project grants (on sphingolipids in cancer at MUSC and apoptosis in liver cells at a collaborating institution), an NIH/NCRR Center of Biomedical Research Excellence (initial and competing renewal grants), and multiple individual investigator research projects as an outstanding core facility of the NCI-designated Hollings Cancer Center at MUSC. In 2008, the Analytic Core Lab logged >14,500 lipid assays for 31 MUSC users, 41 users outside MUSC, and 11 international users. The core lab currently relies on three operational mass spectrometry set ups - all are being used to capacity. Therefore, a core group of 18 major users, representing 8 departments at MUSC, are requesting this grant to acquire a Thermo TSQ Quantum Access mass spectrometer. This request is supported by letters from a representative sample of 6 outside users at biomedical research institutions across the nation. Technical expertise within the Analytic Core Lab includes a fulltime faculty member with extensive qualitative and quantitative lipid chemistry experience and 3 staff members. A comprehensive Administrative Plan ensures appropriate management, oversight, equitable access, and compliance. Strong institutional commitment ensures long term operation and maintenance of the requested instrument and overall facility. PUBLIC HEALTH RELEVANCE: The requested instrument will be used to analyze and quantify specific lipids that are important biological mediators regulating a broad range of cellular processes. Lipids function as both energy stores and signaling molecules. In addition, they form an impermeable bilayer separating the intra- and extracellular components of cells. Major lipid classes include triacylglycerols, phospholipids, glycolipids, sphingolipids, sterols, waxes and fatty acids. Because alteration of lipid function is strongly implicated in the etiology and pathogenesis of many diseases that have a very high burden of personal and social cost, e.g., atherosclerosis, Alzheimer's disease, cancer, type 2 diabetes, inflammatory disorders, infectious diseases, neurodegeneration, obesity and stroke, research in this area is vital for identifying potential therapeutic targets.