Amnis ImageStream System

描述（由申请人提供）：波士顿生物医学研究所（BBRI）的研究人员从事各种各样的研究兴趣，其统一目标是阐明在人类疾病中出错的正常细胞和分子机制。癌症生物学，再生生物学，心血管生物学和综合蛋白质生物学的研究方向是为了实现生物医学和医学疗法的新进展。为了提高生物医学研究项目的质量和范围，BBRI的研究人员正在申请资金购买Amnis公司生产的ImageStream（R）细胞分析仪。这种数字成像流式细胞仪结合了荧光显微镜和流式细胞术的独特功能，提供了许多有利的集成分子细胞分析应用。这些集成应用不能用两种独立的细胞分析方法中的任一种或甚至两种来执行。ImageStream（R）分析仪将放置在监督P.I.的实验室内。根据BBRI的“合作平台”仪器使用方法，经过适当培训后，将向BBRI研究人员提供定期免费访问。私家侦探的实验室人员将负责维护仪器，确保其正确使用，并监测对涉及原代人体组织细胞和人体细胞株的分析的批准机构生物安全协议的正确遵守。BBRI的研究委员会（COR）将提供额外的一般监督和使用审查，该委员会是一个内部科学咨询委员会，由代表BBRI全方位研究兴趣和专业知识的教师组成。BBRI致力于提供支持该仪器的服务合同和运营成本。在获得ImageStream（R）细胞分析仪后，11个BBRI研究项目将实现立即和持续的进展加速。其中包括7名R01级受资助的高级和初级研究者的研究项目，他们将成为主要用户，以及4名次要用户，包括3名新研究者的新兴研究项目。该仪器还将加强总部设在BBRI的Wellstone合作肌肉营养不良研究中心的研究。

DESCRIPTION (provided by applicant): Investigators at Boston Biomedical Research Institute (BBRI) pursue a diverse range of research interests that have the unifying goal of elucidating normal cellular and molecular mechanisms that go awry in human disease. Institute-wide, programmatic research thrusts in Cancer Biology, Regenerative Biology, Cardiovascular Biology, and Integrative Protein Biology are motivated by the intent to enable new advances in biomedicine and medical therapies. To enhance the quality and scope of their biomedical research programs, investigators at BBRI are applying for funds to purchase an ImageStream(R) cell analyzer manufactured by the Amnis Corporation. This digital imaging flow cytometer combines the unique features of fluorescent light microscopy and flow cytometry to provide a number of advantageous integrated molecular cell analysis applications. These integrated applications cannot be performed with either, or even both, of the two independent cell analysis methodologies. The ImageStream(R) analyzer will be housed within the laboratory of the overseeing P.I. Scheduled, free access will be provided to BBRI research personnel after proper training according to BBRI's "platforms for collaboration" instrument usage approach. The P.I.'s lab personnel will be responsible for maintaining the instrument, insuring its proper use, and monitoring proper adherence to approved institutional biosafety protocols for analyses that involve primary human tissue cells and human cell strains. Additional general oversight and usage review will be provided by BBRI's Committee on Research (COR), which is an internal scientific advisory committee composed of faculty who represent the full range of research interests and expertise at BBRI. BBRI is committed to providing service contract and operational costs for support of the instrument. Eleven BBRI research programs will realize an immediate and sustained acceleration of progress upon acquisition of the ImageStream(R) cell analyzer. These include the research programs of 7 R01-level, funded senior and junior investigators, who will be major users, and 4 minor users, including the emerging research programs of 3 new investigators. The instrument will also enhance research in a Wellstone Cooperative Muscular Dystrophy Research Center headquartered at BBRI.