Automated Nucleic Acid Extractor System:AutoGenFlex Star/AutoGen Quick-Gene 810

自动化核酸提取系统：AutoGenFlex Star/AutoGen Quick-Gene 810

• 批准号: 7793989
• 负责人: KENNETH CORNETTA
• 金额: $ 15.95万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-20 至 2011-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793989
• 关键词: Affect; Alzheimer' s Disease; Area; Arts; Bioinformatics; Cancer Center; Cells; Clinical; Clinical Medicine; Clinical Research; Collection; Contract Services; DNA; Development; Disease; Ensure; Equipment; Fees; Fostering; Funding; Genes; Genetic; Grant; Health; Housing; Indiana; Individual; Institutes; Institution; International; Investments; Maintenance; Medicine; National Center for Research Resources; Nucleic Acids; Process; RNA; Rare Diseases; Reagent; Research; Research Personnel; Sampling; Specimen; System; Time; Tissue Banking; Tissue Banks; Translational Research; United States National Institutes of Health; Universities; Work; analytical tool; base; biobank; cost; genetic analysis; improved; instrument; medical schools; member; novel; repository; sample collection; vector

DESCRIPTION (provided by applicant): This request is for funds to purchase a nucleic acid extraction system consisting of an AutoGenFlex Star automated DNA isolation system and the AutoGen Quick-Gene 810 RNA isolation unit. The instruments will be housed, managed, and maintained by the Indiana Clinical and Translational Sciences Institute (CTSI) within the IndianaCTSI Specimen Storage Facility. Biobanking has become an integral part of translational research. The rapid developments in high throughput sequencing along with novel bioinformatic analytical tools has led to an increase in the number of investigators performing novel genetic analysis. With this increase in analytical capacity, the limiting step has become the collection and isolation of nucleic acids. The equipment will facilitate health research for the CTSI member institutions: Indiana University (IU), Purdue University and the University of Notre Dame. All three members of the Indiana CTSI will share a common state-of-the-art repository that was built, in part, through a NCRR construction grant (C06-RR020128-01, 09/30/04 - 09/29/08, R.S. Fife, PI, K. Cornetta, Co-I). The facility will be housed at the IU School of Medicine. In addition to supporting individual investigators, the IU Simon Cancer Center (2P30CA082709), the National Cell Repository for Alzheimer Disease (5U24AG021886; PI: T. Foroud), the National Gene Vector Biorepository (P40 RR024928; PI K. Cornetta), and the Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center work closely with the Indiana CTSI repository efforts. As a result, the instrument system will have an impact on a much larger group of NIH funded investigators than those located in Indiana. The facility will serve as a recharge center for users and the service contract and reagent costs will be included in user fees. The CTSI will provide support for a technician dedicated to repository activities including DNA processing and equipment maintenance. By developing a common facility, the Indiana CTSI will facilitate sample collection and processing, promote sharing of samples, and assure compliance with international biobanking standards. The equipment requested in this proposal will improve consistency of biospecimens, increase capacity, and free up technician time for analytical work. Furthermore, the diverse group of investigators who will utilize this equipment will ensure the investment will facilitate basic and clinical research in almost every area of medicine. PROJECT NARRATIVE: Understanding the genetic basis of disease continues to foster the development of new treatments for both common and rare diseases. Automated extraction of DNA can facilitates genetic analysis, a benefit that will positively affect almost every area of clinical medicine and the capacity to provide RNA will allow Indiana investigators to pursue this rapidly expanding field. The equipment requested will improve the research for R01 funded investigators, national biobanking efforts, and Indiana CTSI investigators engaged in basic and clinical research.

描述(由申请人提供)：本申请是为购买一套核酸提取系统而提出的，该系统由AutoGenFlex Star自动DNA分离系统和Autogen Quick-gene 810RNA分离装置组成。这些仪器将由印第安纳临床和转化科学研究所(CTSI)在印第安纳CTSI标本存储设施内容纳、管理和维护。生物库已经成为翻译研究中不可或缺的一部分。高通量测序的快速发展以及新的生物信息学分析工具导致了执行新基因分析的研究人员的数量增加。随着分析能力的提高，限制步骤变成了收集和分离核酸。该设备将促进CTSI成员机构：印第安纳大学(IU)、普渡大学和圣母大学的健康研究。印第安纳州CTSI的所有三名成员将共享一个共同的最先进的储存库，该储存库部分是通过NCRR建设赠款(C06-RR020128-01，09/30/04-09/29/08，R.S.Fife，Pi，K.Cornetta，Co-I)建立的。该设施将设在伊利诺伊大学医学院。除了支持个人研究人员，印第安纳大学西蒙癌症中心(2P30CA082709)、阿尔茨海默病国家细胞库(5U24AG021886；PI：T.Foroud)、国家基因载体生物库(P40 RR024928；Pi K.Cornetta)和印第安纳州西蒙癌症中心的Susan G.Komen治疗组织库与印第安纳州CTSI存储库的工作密切合作。因此，仪器系统对NIH资助的调查人员的影响将比印第安纳州的调查人员多得多。该设施将作为用户的充电中心，服务合同和试剂成本将包括在用户费用中。CTSI将为一名专门从事DNA处理和设备维护等储存库活动的技术员提供支持。通过开发一个共同设施，印第安纳州CTSI将促进样本收集和处理，促进样本共享，并确保遵守国际生物库标准。本提案中要求的设备将改善生物样品的一致性，增加产能，并腾出技术人员时间进行分析工作。此外，将使用这种设备的不同研究人员群体将确保这笔投资将促进几乎所有医学领域的基础和临床研究。 项目简介：了解疾病的遗传基础继续促进常见病和罕见病新疗法的发展。自动提取DNA可以促进基因分析，这一好处将对临床医学的几乎每个领域产生积极影响，提供RNA的能力将使印第安纳州的研究人员能够追求这一迅速扩大的领域。所要求的设备将改善R01资助的研究人员、国家生物库努力以及从事基础和临床研究的印第安纳州CTSI调查人员的研究。