Automated Nucleic Acid Extractor System:AutoGenFlex Star/AutoGen Quick-Gene 810

自动化核酸提取系统：AutoGenFlex Star/AutoGen Quick-Gene 810

• 批准号: 7793989
• 负责人: KENNETH CORNETTA
• 金额: $ 15.95万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-20 至 2011-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793989
• 关键词: Affect; Alzheimer' s Disease; Area; Arts; Bioinformatics; Cancer Center; Cells; Clinical; Clinical Medicine; Clinical Research; Collection; Contract Services; DNA; Development; Disease; Ensure; Equipment; Fees; Fostering; Funding; Genes; Genetic; Grant; Health; Housing; Indiana; Individual; Institutes; Institution; International; Investments; Maintenance; Medicine; National Center for Research Resources; Nucleic Acids; Process; RNA; Rare Diseases; Reagent; Research; Research Personnel; Sampling; Specimen; System; Time; Tissue Banking; Tissue Banks; Translational Research; United States National Institutes of Health; Universities; Work; analytical tool; base; biobank; cost; genetic analysis; improved; instrument; medical schools; member; novel; repository; sample collection; vector

DESCRIPTION (provided by applicant): This request is for funds to purchase a nucleic acid extraction system consisting of an AutoGenFlex Star automated DNA isolation system and the AutoGen Quick-Gene 810 RNA isolation unit. The instruments will be housed, managed, and maintained by the Indiana Clinical and Translational Sciences Institute (CTSI) within the IndianaCTSI Specimen Storage Facility. Biobanking has become an integral part of translational research. The rapid developments in high throughput sequencing along with novel bioinformatic analytical tools has led to an increase in the number of investigators performing novel genetic analysis. With this increase in analytical capacity, the limiting step has become the collection and isolation of nucleic acids. The equipment will facilitate health research for the CTSI member institutions: Indiana University (IU), Purdue University and the University of Notre Dame. All three members of the Indiana CTSI will share a common state-of-the-art repository that was built, in part, through a NCRR construction grant (C06-RR020128-01, 09/30/04 - 09/29/08, R.S. Fife, PI, K. Cornetta, Co-I). The facility will be housed at the IU School of Medicine. In addition to supporting individual investigators, the IU Simon Cancer Center (2P30CA082709), the National Cell Repository for Alzheimer Disease (5U24AG021886; PI: T. Foroud), the National Gene Vector Biorepository (P40 RR024928; PI K. Cornetta), and the Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center work closely with the Indiana CTSI repository efforts. As a result, the instrument system will have an impact on a much larger group of NIH funded investigators than those located in Indiana. The facility will serve as a recharge center for users and the service contract and reagent costs will be included in user fees. The CTSI will provide support for a technician dedicated to repository activities including DNA processing and equipment maintenance. By developing a common facility, the Indiana CTSI will facilitate sample collection and processing, promote sharing of samples, and assure compliance with international biobanking standards. The equipment requested in this proposal will improve consistency of biospecimens, increase capacity, and free up technician time for analytical work. Furthermore, the diverse group of investigators who will utilize this equipment will ensure the investment will facilitate basic and clinical research in almost every area of medicine. PROJECT NARRATIVE: Understanding the genetic basis of disease continues to foster the development of new treatments for both common and rare diseases. Automated extraction of DNA can facilitates genetic analysis, a benefit that will positively affect almost every area of clinical medicine and the capacity to provide RNA will allow Indiana investigators to pursue this rapidly expanding field. The equipment requested will improve the research for R01 funded investigators, national biobanking efforts, and Indiana CTSI investigators engaged in basic and clinical research.

描述（由申请人提供）：本申请旨在获得资金以购买核酸提取系统，该系统由AutoGenFlex星星自动DNA分离系统和AutoGen Quick-Gene 810 RNA分离装置组成。这些仪器将由印第安纳州临床和转化科学研究所（CTSI）在IndianaCTSI标本储存机构内存放、管理和维护。 生物库已成为转化研究的一个组成部分。高通量测序的快速发展沿着新的生物信息学分析工具，导致进行新的遗传分析的研究者数量增加。随着分析能力的提高，限制性步骤已成为核酸的收集和分离。这些设备将促进CTSI成员机构的健康研究：印第安纳州大学、普渡大学和圣母大学。印第安纳州CTSI的所有三个成员将共享一个共同的最先进的储存库，该储存库部分是通过NCRR建设赠款建造的（C 06-RR 020128 -01，09/30/04 - 09/29/08，R.S.法夫，派，K。Cornetta，Co-I）.该设施将设在IU医学院。除了支持个人研究者，IU西蒙癌症中心（2 P30 CA 082709），国家阿尔茨海默病细胞库（5 U24 AG 021886; PI：T。Foroud）、国家基因载体生物保藏库（P40 RR 024928; PI K. Cornetta）和Susan G.科门的治愈组织银行在国际单位西蒙癌症中心密切合作，与印第安纳州CTSI库的努力。因此，仪器系统将对NIH资助的研究人员产生影响，而不是位于印第安纳州的研究人员。该设施将作为用户的充值中心，服务合同和试剂费用将包含在用户费用中。CTSI将为一名专门从事处置库活动的技术人员提供支持，包括DNA处理和设备维护。通过建立一个共同的设施，印第安纳州CTSI将促进样本收集和处理，促进样本共享，并确保符合国际生物库标准。本提案中要求的设备将提高生物标本的一致性，增加能力，并使技术人员有时间进行分析工作。此外，将使用该设备的多元化研究人员将确保投资将促进几乎所有医学领域的基础和临床研究。 了解疾病的遗传基础将继续促进常见病和罕见病新疗法的开发。DNA的自动提取可以促进遗传分析，这一好处将积极影响临床医学的几乎每一个领域，而提供RNA的能力将使印第安纳州的研究人员能够追求这一迅速扩大的领域。所要求的设备将改善R 01资助的研究人员、国家生物库工作和从事基础和临床研究的印第安纳州CTSI研究人员的研究。