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Molecular Biology and Analytical Core

分子生物学和分析核心

基本信息

批准号：
8055476
负责人：
WILLIAM H BEIERWALTES
金额：
$ 24.21万
依托单位：
HENRY FORD HEALTH SYSTEM
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8055476
关键词：
1-Phosphatidylinositol 3-Kinase 2-Mercaptoethanol Accidents Acetates Acids Adenosine Adenovirus Vector Adenoviruses Adenylate Cyclase Affect Analytical Chemistry Angiotensin I Angiotensinogen Antibodies Arts Back Bathing Binding Biological Biological Assay Biomedical Technology CMV promoter Calcium California Calmodulin Camping Cations Cells Charge Cholates Cities Clinical Chemistry Cloning Color Complementary DNA Computer software Computers Consensus Sequence Crotalus Cultured Cells Cyan Fluorescent Protein Cyclic GMP DNA Data Deoxyribonuclease I Detection Detergents Dose Edetic Acid Egtazic Acid Electrolytes Ensure Enzyme-Linked Immunosorbent Assay Enzymes Equipment Escherichia coli Faculty Firefly Luciferases Fluorescence Resonance Energy Transfer Freedom Freezing Frozen Sections Furuncles Generations Genes Genomics Genotype Glass Glyceraldehyde 3-Phosphate Griess reagent Guanosine Harvest Hospitals Hour Hydrolysis Immunoassay Immunosorbents Incubated Individual Instruction Ion Exchange Ion-Exchange Chromatography Procedure Isoenzymes Juxtaglomerular Cell Kidney Label Laboratories Lasers Learning Letters Leupeptins Light Limb structure Link Liquid substance Location Luciferases Macula densa Mammalian Cell Manuals Manufacturer Name Measurement Measures Membrane Messenger RNA Methods Microscope Modeling Modification Molecular Molecular Analysis Molecular Biology Mus Mutation Nitrate Reductases Nitrates Nitric Oxide Synthase Nitric Oxide Synthase Type I Nitrites Nucleosides One-Step dentin bonding system PDE2 phosphodiesterase Paint Particulate Pepstatins Peptides Peroxidases Phosphatidylinositols Phospho-Specific Antibodies Phosphoric Acids Phosphorylation Phosphoserine Phosphothreonine Phosphotransferases Physiological Plasma Plasmids Preparation Procedures Productivity Proteins Proteomics Proto-Oncogene Proteins c-akt Quality Control RNA Radioimmunoassay Reaction Reader Reading Reagent Recovery Relative (related person)Renal Hypertension Renin Reporter Reporting Research Research Personnel Residual state Resource Sharing Reverse Transcriptase Polymerase Chain Reaction Ribonucleases Rolipram Running Sampling Sepharose Serine Serum Services Shapes Sheep Shipping Ships Shuttle Vectors Signal Transduction Site Small Interfering RNA Snake Venoms Sodium Sodium Chloride Solutions Specimen Staining method Stains Staurosporine Streptavidin Sucrose Sulfanilamide Sulfanilamides Suspension substance Suspensions Tail Techniques Technology Teflon Testing Thick Threonine Time Tissues Toxin Tracer Transfection Transplantation Universities Virginia Water Weight Width Yang adenylyl cyclase 6 animal facility antibody conjugate base chromophore cilostamide cost design detector enzyme activity ethane sulfonate experience gene cloning human NOS3 protein indexing inhibitor/antagonist interest isopentane kidney cortex knockout animal laboratory facility laser capture microdissection leupeptin luciferin medical schools member nitrate reductase oxidation pepstatin phosphodiesterase IV phosphodiesterase V phosphoric diester hydrolase prevent programs promoter receptor research study restriction enzyme sensor sephadex vardenafil vector

项目摘要

The purpose of the Molecular Biology and Analytical Core (Core B) is to centralize molecular analysis, cloning, laser capture microdissection, radioimmunoassays (RIA), enzyme activity assays, and analytical chemistry. Analytical chemistry, radioimmunoassay, enzyme immunosorbent assays, real time RT-PCR and cloning of siRNAs into shuttle vectors are of fundamental importance for state-of-the-art hypertension and renal research. Core B will perform a number of labor-intensive and time-consuming analyses that will relieve investigators from having to conduct these assays themselves and thereby allow them more time to pursue their scientific efforts. The molecular biology aspect of the core will aid investigators by cloning, performing laser capture microdissection, real time RT-PCR and genotyping knockout animals. The analytical aspect of the core will use RIA to measure cAMP and cGMP, and will also determine Ang I as an index of renin activity and/or concentration. Enzyme activity assays will also be performed for phosphodiesterase activity, phosphatidylinositol 3 kinase (PIS kinase) activity, and Akt activity. Analytical chemistry will be performed to determine calcium and sodium concentrations. Centralizing of analytical and molecular studies in Core B will ensure cost efficiency in terms of labor and laboratory facilities/equipment by eliminating duplication of effort by individual investigators. It will also help integrate projects by providing consistent and reliable assays which promote both quality and productivity for participating projects.

分子生物学和分析核心(核心B)的目的是集中分子分析，克隆、激光捕获显微切割、放射免疫分析(RIA)、酶活性分析和分析化学反应。分析化学、放射免疫分析、酶免疫分析、实时RT-PCR和将siRNA克隆到穿梭载体中对于研究最先进的高血压和肾脏研究。核心B将执行大量劳动密集型和耗时的分析，这些分析将使调查人员不必自己进行这些化验，从而使他们有更多的时间继续他们的科学努力。核心的分子生物学方面将通过克隆来帮助研究人员，进行激光捕获显微切割、实时RT-PCR和基因敲除动物的基因分型。这个核心的分析方面将使用RIA来测量cAMP和cGMP，并将确定Ang I作为肾素活性和/或浓度指数。还将进行酶活性分析，以磷酸二酯酶活性、磷脂酰肌醇3激酶(PIS激酶)活性和Akt活性。分析性将进行化学检测以确定钙和钠的浓度。集中分析和核心B的分子研究将通过以下方式确保在劳动力和实验室设施/设备方面的成本效益消除个别调查人员的重复工作。它还将通过提供一致和可靠的分析，提高参与项目的质量和生产率。